Blank S E, Duncan D A, Meadows G G
College of Pharmacy, Washington State University, Pullman 99164-6510.
Alcohol Clin Exp Res. 1991 Feb;15(1):16-22. doi: 10.1111/j.1530-0277.1991.tb00514.x.
Effects of 4-week food restriction and ethanol consumption on natural killer (NK) cell activity and carcass composition were evaluated. Female, C57BL/6 mice given water (H2O) or ethanol (20% w/v, ETOH) ad libitum were placed in one of three dietary groups: unrestricted (UNR), moderately restricted (MR, 2.2 g/day), or severely restricted (SR, 1.8 g/day). Food restriction alone (MR, SR) significantly reduced body, spleen, and thymus weights; carcass lipid content (SR only); spleen cell number; and baseline and interleukin-2 (rIL-2) stimulated NK cell activities. Ethanol consumption was unaffected by food restriction and in restricted mice it did not suppress food intake. Thus, average calories derived from ethanol increased from 30% (UNR) to 40% (SR) with the degree of food restriction in these groups. Mice given ethanol and restricted food intake had at least as heavy or heavier body, spleen, and thymus weights than water-drinking (H2O) counterparts. Spleen cell number was reduced in ethanol-consuming (ETOH), food restricted groups compared with UNR H2O control. Baseline NK cell activity was suppressed 50% to 90% in all ETOH and food-restricted groups. rIL-2 stimulated NK cell activity was suppressed 18% to 76% in food restricted mice independent of ethanol intake. These results indicate that supplementary ethanol calories did not enhance NK cell activity in UNR ETOH mice, nor did they protect splenic NK cell activity from the suppressant effects of food restriction. Ethanol consumption significantly increased carcass lipid content in all groups compared with their H2O counterparts. This increase was largely responsible for the preservation of body weight in ETOH mice especially during food restriction.
评估了为期4周的食物限制和乙醇摄入对自然杀伤（NK）细胞活性及胴体组成的影响。将随意饮用清水（H2O）或乙醇（20% w/v，ETOH）的雌性C57BL/6小鼠分为三个饮食组之一：无限制饮食组（UNR）、适度限制饮食组（MR，2.2克/天）或严重限制饮食组（SR，1.8克/天）。单独的食物限制（MR、SR）显著降低了体重、脾脏和胸腺重量；胴体脂质含量（仅SR组）；脾细胞数量；以及基础和白细胞介素-2（rIL-2）刺激的NK细胞活性。乙醇摄入不受食物限制的影响，在限制饮食的小鼠中，乙醇摄入也不抑制食物摄入量。因此，随着这些组食物限制程度的增加，来自乙醇的平均卡路里从30%（UNR组）增加到40%（SR组）。给予乙醇且食物摄入量受限的小鼠，其体重、脾脏和胸腺重量至少与饮用清水（H2O）的对照组相当或更重。与UNR H2O对照组相比，摄入乙醇且食物受限的组中脾细胞数量减少。在所有ETOH组和食物限制组中，基础NK细胞活性被抑制了50%至90%。在食物受限的小鼠中，无论乙醇摄入量如何，rIL-2刺激的NK细胞活性被抑制了18%至76%。这些结果表明，补充乙醇卡路里并未增强UNR ETOH小鼠的NK细胞活性，也未保护脾脏NK细胞活性免受食物限制的抑制作用。与饮用清水的对应组相比，所有组中乙醇摄入均显著增加了胴体脂质含量。这种增加在很大程度上导致了ETOH小鼠体重的维持，尤其是在食物限制期间。
