Lawton L A, Edwards C, Codd G A
Department of Biological Sciences, University of Dundee, UK.
Analyst. 1994 Jul;119(7):1525-30. doi: 10.1039/an9941901525.
Increasing concern over the presence of microcystins (cyanobacterial/blue-green algal hepatotoxins) in water supplies has emphasized the need for a suitable analytical method. As many microcystins are known to exist, a method was developed that permits the determination of numerous variants by a single procedure. The method involves filtration to separate cyanobacterial cells from water, allowing intracellular and extracellular toxin levels to be assessed. The cellular components of the samples are extracted repeatedly in methanol, which was found to be the most versatile solvent tested for the extraction of microcystins. The efficiency of this extraction procedure was found to be independent of cell biomass. The filtered water was subjected to trace enrichment using a C18 solid-phase extraction cartridge, followed by identification and determination by photodiode-array high-performance liquid chromatography. The procedure was assessed using four water samples (two raw and two treated) spiked with a mixture of five microcystins and the cyanobacterial hepatotoxin nodularin. Recoveries of all but one microcystin were found to be good when spiked with concentrations as low as 250 ng l-1. The linearity and precision of the experimental procedure were assessed for five microcystins and nodularin. The proposed method permits rapid sample processing and determination of several microcystins.
人们对供水系统中微囊藻毒素（蓝藻/蓝绿藻肝毒素）的存在日益关注，这凸显了需要一种合适的分析方法。由于已知存在多种微囊藻毒素，因此开发了一种方法，可通过单一程序测定多种变体。该方法包括过滤以将蓝藻细胞与水分离，从而能够评估细胞内和细胞外毒素水平。样品的细胞成分用甲醇反复萃取，结果发现甲醇是测试过的用于萃取微囊藻毒素的最通用溶剂。发现这种萃取程序的效率与细胞生物量无关。过滤后的水使用C18固相萃取柱进行痕量富集，然后通过光电二极管阵列高效液相色谱进行鉴定和测定。使用四个加标了五种微囊藻毒素和蓝藻肝毒素节球藻毒素混合物的水样（两个原水和两个处理后水）对该程序进行了评估。当加标浓度低至250 ng l-1时，除一种微囊藻毒素外，其他所有微囊藻毒素的回收率都很好。对五种微囊藻毒素和节球藻毒素的实验程序的线性和精密度进行了评估。所提出的方法允许快速进行样品处理并测定几种微囊藻毒素。
