Center for Water Quality and Algae Research, Department of Zoology, University of Sri Jayewardenepura, Gangodawila, Nugegoda 10250, Sri Lanka.
Toxins (Basel). 2019 Jul 24;11(8):432. doi: 10.3390/toxins11080432.
The potential transfer of microcystin-LR (MC-LR) to humans via crop plants irrigated with MC-contaminated water is causing serious concern. In this study, two variants, a hybrid (BG358), a traditional (Suwandel) variety, and a leafy green vegetable crop, , were exposed under laboratory conditions to natural blooms of sampled from a hypereutrophic lake contaminated with MC-LR (3,197.37 ± 1.04 µg/L). Field samples of and were collected from farmlands that had been irrigated from a reservoir, containing MC-LR (180 µg/L). MC-LR was quantified by high performance liquid chromatography followed by photodiode-array detection (HPLC-PDA). From the laboratory study, we calculated the potential human health exposure from BG358, Suwandel and as 2.84 ± 0.01, 0.22 ± 0.01, and 0.06 ± 0.01 µg/kg of body weight/day, respectively, whereas the potential health exposures from BG358, Suwandel and collected from the field were 0.10 ± 0.01, 0.009 ± 0.005, and 0.03 ± 0.01 µg/kg of body weight/day, respectively. In certain instances, the results exceeded the World Health Organization's (WHO) tolerable daily intake of MC-LR, posing a potential health risk to humans. Thus, our results emphasize the importance of continuous screening programs for cyanotoxins in edible plants in the future to prevent the consumption of contaminated crops.
受微囊藻毒素-LR（MC-LR）污染的水灌溉作物，其向人类转移的潜在风险引起了人们的高度关注。在本研究中，两种变异体，杂交种（BG358）和传统品种（Suwandel），以及一种绿叶蔬菜作物，分别在实验室条件下暴露于从富营养化湖泊中采集的自然藻华样本中，该湖泊被 MC-LR 污染（3,197.37 ± 1.04 µg/L）。从灌溉水库的农田中采集了 和 田间样本，该水库含有 MC-LR（180 µg/L）。通过高效液相色谱法结合光电二极管阵列检测（HPLC-PDA）定量测定 MC-LR。从实验室研究中，我们计算出 BG358、Suwandel 和 对人体健康的潜在暴露量分别为 2.84 ± 0.01、0.22 ± 0.01 和 0.06 ± 0.01 µg/kg体重/天，而从田间采集的 BG358、Suwandel 和 对人体健康的潜在暴露量分别为 0.10 ± 0.01、0.009 ± 0.005 和 0.03 ± 0.01 µg/kg体重/天。在某些情况下，结果超过了世界卫生组织（WHO）对 MC-LR 的可耐受日摄入量，对人类健康构成潜在威胁。因此，我们的研究结果强调了未来在食用植物中进行持续的蓝藻毒素筛查计划的重要性，以防止食用受污染的作物。
