Agaba M, Kemp S J
ILRAD, Nairobi, Kenya.
Anim Genet. 1994 Jun;25(3):187-9. doi: 10.1111/j.1365-2052.1994.tb00109.x.
We have identified substitutions in the 3' untranslated region of the bovine myoglobin gene, one of which affects an MboII restriction enzyme site resulting in a bi-allelic restriction fragment length polymorphism. Co-dominant inheritance of the alleles in three reference families was observed using a polymerase chain reaction--restriction fragment length polymorphism assay. The distribution of the alleles seems characteristic of cattle type--one of the alleles was not detected in purely taurine breeds. Furthermore, we mapped, using the polymerase chain reaction on a bovine-rodent somatic cell hybrid panel, the myoglobin gene to bovine chromosome five. It is therefore syntenic with gamma-interferon and insulin-like growth factor in which we have not found polymorphism. The myoglobin locus therefore serves as a type one marker on bovine chromosome five.
我们已经在牛肌红蛋白基因的3'非翻译区鉴定出了替换，其中一个替换影响了MboII限制性酶切位点，导致双等位基因限制性片段长度多态性。使用聚合酶链反应-限制性片段长度多态性分析，在三个参考家系中观察到了等位基因的共显性遗传。等位基因的分布似乎具有牛品种的特征——在纯牛品种中未检测到其中一个等位基因。此外，我们使用聚合酶链反应在牛-啮齿动物体细胞杂种面板上，将肌红蛋白基因定位到牛的5号染色体上。因此，它与γ-干扰素和胰岛素样生长因子同线，我们在其中未发现多态性。因此，肌红蛋白基因座是牛5号染色体上的一类标记。
