Datta J, Dutta T K, Samanta T B
Department of Microbiology, Bose Institute, Calcutta, India.
Biochem Biophys Res Commun. 1994 Sep 30;203(3):1508-14. doi: 10.1006/bbrc.1994.2356.
Induction of microsomal glutathione S-transferase isozymes in Aspergillus ochraceus TS is reported. Among the inducers of mammalian drug-metabolising enzymes 3-methylcholanthrene, benzo(a)pyrene and polychlorinated biphenyl enhanced the microsomal GST activity quite considerably. On the other hand phenobarbital, beta-naphthoflavone and trans-stilbene oxide were almost without any effect. To our knowledge this seems to be the first report on induction of microsomal GST in lower eukaryotes. A significant increase (7.9 fold) in GST activity was observed with the cells induced by 3-methylcholanthrene. Moreover, three new GST isozymes were found to be present in 3-methylcholanthrene induced microsomes. The isozymes are basic in nature. The GST isozymes were found to be catalytically distinct from each other.
据报道，在赭曲霉TS中可诱导微粒体谷胱甘肽S-转移酶同工酶。在哺乳动物药物代谢酶的诱导剂中，3-甲基胆蒽、苯并(a)芘和多氯联苯相当显著地增强了微粒体谷胱甘肽S-转移酶（GST）的活性。另一方面，苯巴比妥、β-萘黄酮和反式氧化芪几乎没有任何作用。据我们所知，这似乎是关于在低等真核生物中诱导微粒体谷胱甘肽S-转移酶的首次报道。用3-甲基胆蒽诱导的细胞中观察到谷胱甘肽S-转移酶活性显著增加（7.9倍）。此外，在3-甲基胆蒽诱导的微粒体中发现存在三种新的谷胱甘肽S-转移酶同工酶。这些同工酶本质上是碱性的。发现谷胱甘肽S-转移酶同工酶彼此之间具有不同的催化作用。
