Ichikawa K, Khamashta M A, Koike T, Matsuura E, Hughes G R
The Rayne Institute, St. Thomas' Hospital, London, UK.
Arthritis Rheum. 1994 Oct;37(10):1453-61. doi: 10.1002/art.1780371008.
To elucidate the specificity of anticardiolipin antibodies (aCL) from patients with the antiphospholipid syndrome (APS) to various phospholipids (PLs), DNA, and beta 2-glycoprotein I (beta 2-GPI).
Five monoclonal aCL were established from peripheral blood lymphocytes of 3 patients with the APS. The reactivity of monoclonal aCL with various PLs, with DNA, and with beta 2-GPI was examined by enzyme-linked immunosorbent assay (ELISA).
All of the monoclonal aCL bound to anionic PLs, only in the presence of beta 2-GPI. Neither monoclonal aCL nor beta 2-GPI bound to DNA. Monoclonal aCL bound to solid-phase beta 2-GPI on polystyrene ELISA plates that had carboxyl groups on their surface, but did not react with solid-phase beta 2-GPI on ordinary polystyrene plates. A mixture of beta 2-GPI and CL inhibited the binding of monoclonal aCL to beta 2-GPI, but CL or beta 2-GPI alone did not.
Monoclonal aCL may recognize a cryptic epitope, which appears as a result of beta 2-GPI binding to anionic PLs or to polystyrene with carboxyl groups.
阐明抗磷脂综合征（APS）患者的抗心磷脂抗体（aCL）对各种磷脂（PL）、DNA和β2-糖蛋白I（β2-GPI）的特异性。
从3例APS患者的外周血淋巴细胞中建立了5种单克隆aCL。通过酶联免疫吸附测定（ELISA）检测单克隆aCL与各种PL、DNA和β2-GPI的反应性。
所有单克隆aCL仅在β2-GPI存在的情况下与阴离子PL结合。单克隆aCL和β2-GPI均不与DNA结合。单克隆aCL与表面带有羧基的聚苯乙烯ELISA板上的固相β2-GPI结合，但不与普通聚苯乙烯板上的固相β2-GPI反应。β2-GPI和CL的混合物可抑制单克隆aCL与β2-GPI的结合，但单独的CL或β2-GPI则不能。
单克隆aCL可能识别一种隐蔽表位，该表位是由于β2-GPI与阴离子PL或带有羧基的聚苯乙烯结合而出现的。
