• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

利用人细胞提取物对超螺旋类核DNA中氧化和紫外线诱导的DNA损伤进行体外修复。

In vitro repair of oxidative and ultraviolet-induced DNA damage in supercoiled nucleoid DNA by human cell extract.

作者信息

Collins A R, Fleming I M, Gedik C M

机构信息

Boyd Orr Research Centre, Rowett Research Institute, Bucksburn, Aberdeen, UK.

出版信息

Biochim Biophys Acta. 1994 Nov 22;1219(3):724-7. doi: 10.1016/0167-4781(94)90236-4.

DOI:10.1016/0167-4781(94)90236-4
PMID:7948034
Abstract

A human cell extract is capable of carrying out damage-specific incision on supercoiled nucleoid DNA. The DNA breaks are detected by single cell gel electrophoresis. Incubation of the incised DNA with extract and DNA precursors results in resealing of the breaks, with retention of the supercoiled state of the DNA.

摘要

人细胞提取物能够对超螺旋类核DNA进行损伤特异性切割。通过单细胞凝胶电泳检测DNA断裂情况。将切割后的DNA与提取物及DNA前体一起孵育，结果是断裂处重新封闭，DNA保持超螺旋状态。

相似文献

1
In vitro repair of oxidative and ultraviolet-induced DNA damage in supercoiled nucleoid DNA by human cell extract.利用人细胞提取物对超螺旋类核DNA中氧化和紫外线诱导的DNA损伤进行体外修复。
Biochim Biophys Acta. 1994 Nov 22;1219(3):724-7. doi: 10.1016/0167-4781(94)90236-4.
2
Repair of quercetin-induced single-strand breaks by a cell free system.利用无细胞系统修复槲皮素诱导的单链断裂
Biochem Mol Biol Int. 1994 Feb;32(2):341-7.
3
Radiation-induced damage in two human glioma cell lines as measured by the nucleoid assay.通过核仁分析法测定两种人胶质瘤细胞系中的辐射诱导损伤。
Anticancer Res. 1997 Nov-Dec;17(6D):4615-8.
4
Differences in the photogenotoxic potential of two fluoroquinolones as shown in diploid yeast strain (Saccharomyces cerevisae) and supercoiled plasmid DNA.两种氟喹诺酮类药物在二倍体酵母菌株（酿酒酵母）和超螺旋质粒DNA中表现出的光遗传毒性潜力差异。
Mutat Res. 2000 Jun 22;468(1):1-9. doi: 10.1016/s1383-5718(00)00034-6.
5
The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells.
Mutat Res. 1995 Jan;336(1):69-77. doi: 10.1016/0921-8777(94)00043-6.
6
Single-strand breaks and repair in nuclear DNA in the presence of hydralazine assayed by the nucleoid technique.采用核仁技术检测在肼存在的情况下核DNA中的单链断裂及修复情况。
Biochem Mol Biol Int. 1997 Oct;43(3):513-9. doi: 10.1080/15216549700204311.
7
Workshop on single cell gel electrophoresis (the 'comet assay') held as part of the UKEMS/DNA Repair Network Joint Meeting, Swansea, March 1992.作为1992年3月在斯旺西举行的英国环境诱变剂学会/DNA修复网络联席会议的一部分举办的单细胞凝胶电泳（“彗星试验”）研讨会。
Mutagenesis. 1992 Sep;7(5):357-8. doi: 10.1093/mutage/7.5.357.
8
Single-strand breaks in supercoiled DNA induced by vacuum-UV radiation in aqueous solution.水溶液中真空紫外线辐射诱导超螺旋DNA产生的单链断裂。
Photochem Photobiol. 1986 Sep;44(3):397-400. doi: 10.1111/j.1751-1097.1986.tb04682.x.
9
Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA.人淋巴细胞DNA中内源性氧化碱基损伤的直接酶促检测
Carcinogenesis. 1993 Sep;14(9):1733-5. doi: 10.1093/carcin/14.9.1733.
10
Photosensitized damage to supercoiled plasmid DNA induced by 334-nm radiation in the presence of 2-thiouracil consists of alkali- and piperidine-labile sites as well as frank strand breaks.在2-硫尿嘧啶存在的情况下，334纳米辐射诱导的超螺旋质粒DNA的光致敏损伤包括碱不稳定位点、哌啶不稳定位点以及明显的链断裂。
Photochem Photobiol. 1990 Nov;52(5):1017-23. doi: 10.1111/j.1751-1097.1990.tb01819.x.

引用本文的文献

1
Comparison of comet-based approaches to assess base excision repair.彗星实验方法评估碱基切除修复的比较。
Arch Toxicol. 2023 Aug;97(8):2273-2281. doi: 10.1007/s00204-023-03543-y. Epub 2023 Jun 22.
2
Titanium biogenic nanoparticles to help the growth of Trichoderma harzianum to be used in biological control.钛生物生成纳米颗粒帮助哈茨木霉生长，以用于生物防治。
J Nanobiotechnology. 2023 May 25;21(1):166. doi: 10.1186/s12951-023-01918-y.
3
Polymeric Nanorepellent Systems Containing Geraniol and Icaridin Aimed at Repelling .含有香叶醇和驱蚊酯的聚合物纳米拒斥系统旨在驱蚊。
Int J Mol Sci. 2022 Jul 27;23(15):8317. doi: 10.3390/ijms23158317.
4
The regulation of DNA supercoiling across evolution.DNA 超螺旋结构在进化中的调控。
Protein Sci. 2021 Oct;30(10):2042-2056. doi: 10.1002/pro.4171. Epub 2021 Aug 23.
5
Influence of the capping of biogenic silver nanoparticles on their toxicity and mechanism of action towards Sclerotinia sclerotiorum.生物合成银纳米粒子的帽化对其毒性的影响及对核盘菌的作用机制。
J Nanobiotechnology. 2021 Feb 24;19(1):53. doi: 10.1186/s12951-021-00797-5.
6
An optimized comet-based in vitro DNA repair assay to assess base and nucleotide excision repair activity.一种优化的基于彗星的体外 DNA 修复检测方法，用于评估碱基和核苷酸切除修复活性。
Nat Protoc. 2020 Dec;15(12):3844-3878. doi: 10.1038/s41596-020-0401-x. Epub 2020 Nov 16.
7
A Multi-Endpoint Approach to Base Excision Repair Incision Activity Augmented by PARylation and DNA Damage Levels in Mice: Impact of Sex and Age.多靶点方法评估 PAR 化和 DNA 损伤水平对小鼠碱基切除修复切口活性的影响：性别和年龄的作用。
Int J Mol Sci. 2020 Sep 9;21(18):6600. doi: 10.3390/ijms21186600.
8
Synthesis of biogenic silver nanoparticles using Althaea officinalis as reducing agent: evaluation of toxicity and ecotoxicity.采用药用蜀葵作为还原剂合成生物银纳米粒子：毒性和生态毒性评价。
Sci Rep. 2018 Aug 17;8(1):12397. doi: 10.1038/s41598-018-30317-9.
9
The Cellular Response to Oxidatively Induced DNA Damage and Polymorphism of Some DNA Repair Genes Associated with Clinicopathological Features of Bladder Cancer.细胞对氧化诱导的DNA损伤的反应以及与膀胱癌临床病理特征相关的一些DNA修复基因的多态性
Oxid Med Cell Longev. 2016;2016:5710403. doi: 10.1155/2016/5710403. Epub 2015 Nov 16.
10
The comet assay: past, present, and future.彗星试验：过去、现在与未来。
Front Genet. 2015 Aug 13;6:266. doi: 10.3389/fgene.2015.00266. eCollection 2015.