Aretio R, Pérez M J, Martín E
Sección de Microbiología, Hospital Universitario de Valme, Sevilla.
Enferm Infecc Microbiol Clin. 1994 Aug-Sep;12(7):337-40.
Two immunology techniques (ELISA and DIF) were evaluated for the detection of Giardia lamblia in faeces, using microscopic examination as reference method.
Stool specimens from 378 patients were studied, in our laboratory for detection of G. lamblia. All were examined visually by microscope after carrying out the concentration technique with Para-Pak Macro-Con (Meridian Diagnostics, Cincinnati, Ohio). The ELISA technique (Giardia Celisa Test Cellabs Diagnostics PTY LTD, Brookvale, Australia) was used on the 378 faeces specimens and 294 of them were processed also by DIF (Giardia-Cel IF Test, from the same commercial supplier).
A total of 16 positive visual detections was obtained. Bot the ELISA and DIF techniques showed an excellent sensitivity (100% for both) and specificity (97.5% and 98.5%) respectively, as well as a negative predictive value of 100% for ELISA and DIF, whilst the positive predictive value is reduced to 79% in DIF and 64% in ELISA.
From our point of view, both tests are of utility for the screening of carriers, for epidemiological analyses, for the study of outbreaks of diarrhoea due to contaminated waters, as well as non-invasive methods for diagnosis in suggestive clinics with negative microscopic examinations.
以显微镜检查作为参考方法，评估了两种免疫学技术（酶联免疫吸附测定法和直接免疫荧光法）用于检测粪便中蓝氏贾第鞭毛虫的情况。
对378例患者的粪便标本进行研究，在我们实验室检测蓝氏贾第鞭毛虫。在用Para-Pak Macro-Con（Meridian Diagnostics公司，俄亥俄州辛辛那提）进行浓缩技术处理后，所有标本均通过显微镜进行目视检查。对378份粪便标本使用酶联免疫吸附测定技术（蓝氏贾第鞭毛虫酶联免疫吸附试验，Cellabs Diagnostics PTY LTD公司，澳大利亚布鲁克维尔），其中294份标本还采用直接免疫荧光法（蓝氏贾第鞭毛虫直接免疫荧光试验，来自同一商业供应商）进行处理。
共获得16例阳性目视检测结果。酶联免疫吸附测定法和直接免疫荧光法均显示出极佳的敏感性（两者均为100%）和特异性（分别为97.5%和98.5%），酶联免疫吸附测定法和直接免疫荧光法的阴性预测值均为100%，而直接免疫荧光法的阳性预测值降至79%，酶联免疫吸附测定法的阳性预测值降至64%。
在我们看来，这两种检测方法对于携带者筛查、流行病学分析、受污染水源导致的腹泻暴发研究以及在显微镜检查呈阴性的疑似临床病例中作为非侵入性诊断方法均有用处。
