Torres D, Fernández M, Brito T, Finlay C
Instituto de Medicina Tropical Pedro Kourí.
Rev Cubana Med Trop. 1997;49(1):52-8.
An ELISA sandwich for the detection of Giardia lamblia antigens in human faeces was standardized. 175 samples were studied: 77 positive, 61 negative to cysts and/or trophozoites by direct faeces test, and 19 positive to other parasite different from G. lamblia. The sensitivity of the technique was 94.8% and the specificity 98.3%. The method detects an antigen concentration of 31 ng. The procedure is simple, sensitive and specific so, it may be useful for diagnosis and in epidemiological studies.
一种用于检测人粪便中贾第虫兰氏贾第鞭毛虫抗原的酶联免疫吸附测定（ELISA）夹心试验被标准化。研究了175份样本：通过直接粪便检测，77份对包囊和/或滋养体呈阳性，61份呈阴性，19份对除兰氏贾第鞭毛虫之外的其他寄生虫呈阳性。该技术的灵敏度为94.8%，特异性为98.3%。该方法可检测到31纳克的抗原浓度。该程序简单、灵敏且特异，因此可能对诊断和流行病学研究有用。
