一种用于测量囊泡膜弯曲模量的新型微量移液管方法。

A novel micropipet method for measuring the bending modulus of vesicle membranes.

作者信息

Zhelev D V, Needham D, Hochmuth R M

机构信息

Department of Mechanical Engineering and Materials Science, Duke University, Durham, North Carolina 27708-0300.

出版信息

Biophys J. 1994 Aug;67(2):720-7. doi: 10.1016/S0006-3495(94)80530-2.

DOI:10.1016/S0006-3495(94)80530-2

PMID:7948685

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1225415/

Abstract

A theoretical model and an experiment are presented for determining the bending modulus of a bilayer vesicle membrane. The vesicle is held with a pipet having a radius between 1 and 2 microns, and the tension in the membrane is changed by changing the suction pressure. Then the vesicle membrane is deformed by aspirating it into a smaller pipet having a radius on the order of 0.5 microns. The relationship between the suction pressures in the two pipets is found to be linear, as predicted by the theoretical model. The curvature of the vesicle membrane at the pipet orifice and the bending modulus are found with the help of the model from the slope and the intercept of the linear experimental relationship between the suction pressures in the two pipets. The bending modulus for the two SOPC membranes studied in these experiments was found to be either 0.6 or 1.15 x 10(-19) J, which is similar to the values measured previously.

摘要

本文提出了一种用于测定双层囊泡膜弯曲模量的理论模型和实验方法。使用半径在1至2微米之间的移液管固定囊泡，并通过改变抽吸压力来改变膜中的张力。然后，将囊泡吸入半径约为0.5微米的较小移液管中，使囊泡膜变形。正如理论模型所预测的那样，发现两个移液管中的抽吸压力之间的关系是线性的。借助该模型，根据两个移液管中抽吸压力之间的线性实验关系的斜率和截距，得出移液管口处囊泡膜的曲率和弯曲模量。在这些实验中研究的两种SOPC膜的弯曲模量为0.6或1.15×10^(-19) J，这与之前测量的值相似。