长链脂肪酸与酰基载体蛋白的激活作用：大肠杆菌中一种新酶——酰基-酰基载体蛋白合成酶的证明。
Activation of long chain fatty acids with acyl carrier protein: demonstration of a new enzyme, acyl-acyl carrier protein synthetase, in Escherichia coli.
作者信息
Ray T K, Cronan J E
出版信息
Proc Natl Acad Sci U S A. 1976 Dec;73(12):4374-8. doi: 10.1073/pnas.73.12.4374.
Abstract
A soluble enzyme activity which catalyzes the synthesis of acyl-acyl carrier protein from acyl carrier proteins, a long chain fatty acid, and ATP has been demonstrated in E. coli. The reaction requires high concentrations of both Ca++ and Mg++ for activity, and cleaves ATP to AMP and PPi. The fatty acyl product has been identified as acyl-acyl carrier protein by its solubility, thioester linkage, molecular weight, charge, and biological activity. Several criteria indicate the enzyme is distinct from acyl-CoA synthetase. The fatty acid specificity of the enzyme suggests a role of acyl-acyl carrier protein synthetase in the incorporation of fatty acids into phospholipid.
摘要
在大肠杆菌中已证实存在一种可溶性酶活性，它能催化由酰基载体蛋白、长链脂肪酸和ATP合成酰基 - 酰基载体蛋白。该反应需要高浓度的Ca++和Mg++才能具有活性，并将ATP裂解为AMP和PPi。通过其溶解性、硫酯键、分子量、电荷和生物活性，已将脂肪酸产物鉴定为酰基 - 酰基载体蛋白。几个标准表明该酶与酰基辅酶A合成酶不同。该酶的脂肪酸特异性表明酰基 - 酰基载体蛋白合成酶在脂肪酸掺入磷脂中的作用。
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本文引用的文献
1
Acetylornithinase of Escherichia coli: partial purification and some properties.大肠杆菌的乙酰鸟氨酸酶：部分纯化及某些性质
J Biol Chem. 1956 Jan;218(1):97-106.
2
Comparative aspects of fatty acid activation in Escherichia coli and Clostridium butyricum.大肠杆菌和丁酸梭菌中脂肪酸活化的比较研究
FEBS Lett. 1969 Feb;2(4):213-216. doi: 10.1016/0014-5793(69)80022-0.
3
Involvement of acyl carrier protein in acylation of glycerol 3-phosphate in Clostridium butyricum. I. Purification of Clostridium butyricum acyl carrier protein and synthesis of long chain acyl derivatives of acyl carrier protein.丁酸梭菌中酰基载体蛋白参与3-磷酸甘油的酰化作用。I. 丁酸梭菌酰基载体蛋白的纯化及酰基载体蛋白长链酰基衍生物的合成。
J Biol Chem. 1967 Oct 10;242(19):4459-65.
4
An improved method for thin-layer chromatography of nucleotide mixtures containing 32P-labelled orthophosphate.一种用于含32P标记正磷酸盐的核苷酸混合物的薄层色谱改进方法。
J Chromatogr. 1969 Mar 11;40(1):103-9. doi: 10.1016/s0021-9673(01)96624-5.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Acyl carrier protein. XV. Studies of -ketoacyl-acyl carrier protein synthetase.酰基载体蛋白。十五。β-酮脂酰-酰基载体蛋白合成酶的研究。
J Biol Chem. 1972 May 25;247(10):3190-8.
7
Correlation of in vivo and in vitro phase transitions of membrane lipids in Escherichia coli.大肠杆菌中膜脂体内外相变的相关性
Proc Natl Acad Sci U S A. 1970 Oct;67(2):606-12. doi: 10.1073/pnas.67.2.606.
8
Fatty acid degradation in Escherichia coli. An inducible system for the uptake of fatty acids and further characterization of old mutants.大肠杆菌中的脂肪酸降解。脂肪酸摄取的诱导系统及旧突变体的进一步表征。
Eur J Biochem. 1971 Apr;19(3):442-50. doi: 10.1111/j.1432-1033.1971.tb01334.x.
9
10
Partial purification and properties of acyl-CoA synthetase of Escherichia coli.大肠杆菌酰基辅酶A合成酶的部分纯化及性质
Eur J Biochem. 1970 Feb;12(3):576-82. doi: 10.1111/j.1432-1033.1970.tb00889.x.
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