大肠杆菌条件致死性rho突变体的分离与鉴定
Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.
作者信息
Inoko H, Shigesada K, Imai M
出版信息
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1162-6. doi: 10.1073/pnas.74.3.1162.
Abstract
Temperature-sensitive nitA (rho) mutants of E. coli were isolated; one of them was characterized as an amber mutant. These strains show the Nit phenotype (transcription of phage lambda DNA independent of the N gene) at low temperatures and are inviable at high temperatures. The mutated sites appear to be between cya and metE on the chromosome. Temperature-sensitive nitA bacteria not only permit leftward transcription of the lambda genome at a high rate in the absence of the lambda N protein, but also allow lambda growth at low temperatures. At high temperatures, phages lambda and T4 are incapable of normal development in these cells, while growth of T7 is not affected. The production of thermally unstable rho by the nitA temperature-sensitive mutant suggests that nitA is the structural gene for rho.
摘要
分离出了大肠杆菌对温度敏感的nitA（rho）突变体；其中一个被鉴定为琥珀突变体。这些菌株在低温下表现出Nit表型（噬菌体λDNA的转录独立于N基因），在高温下无法存活。突变位点似乎位于染色体上的cya和metE之间。对温度敏感的nitA细菌不仅在没有λN蛋白的情况下允许λ基因组向左高速转录，而且在低温下也允许λ生长。在高温下，噬菌体λ和T4在这些细胞中无法正常发育，而T7的生长不受影响。nitA温度敏感突变体产生热不稳定的rho，这表明nitA是rho的结构基因。
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