Fawcett T, Simon W J, Swinhoe R, Shanklin J, Nishida I, Christie W W, Slabas A R
Department of Biological Sciences, University of Durham, UK.
Plant Mol Biol. 1994 Oct;26(1):155-63. doi: 10.1007/BF00039528.
The expression of mRNA and the steady-state levels of two-component enzymes of plant fatty acid synthetase (FAS) were studied. Northern analysis of enoyl-ACP reductase (ER) and stearoyl-ACP desaturase (SD) gene expression showed that steady-state levels of both transcripts increase during lipid deposition in the seed reaching a maximum at 29 days after flowering (DAF). The steady-state level of ER message falls very quickly after reaching its maximum, whereas the SD message is longer-lived. The levels of these specific mRNAs in seed are 15-30 times greater than in leaf. Optimum mRNA expression precedes the maximum levels of synthesis of the two proteins, which in turn precede the maximum level of oil. The expression of isoenzymes of ER were examined by two-dimensional western blotting in both leaf and seed tissue. Four enzymes are expressed in both of these tissues; the two most abundant isoforms in seed material are also the most abundant in leaf tissue.
研究了植物脂肪酸合成酶（FAS）的mRNA表达及双组分酶的稳态水平。对烯酰-ACP还原酶（ER）和硬脂酰-ACP去饱和酶（SD）基因表达的Northern分析表明，在种子脂质沉积过程中，两种转录本的稳态水平均升高，在开花后29天（DAF）达到最大值。ER信息的稳态水平在达到最大值后迅速下降，而SD信息的寿命更长。种子中这些特定mRNA的水平比叶片中的高15 - 30倍。最佳mRNA表达先于两种蛋白质合成的最大水平，而这又先于油的最大水平。通过二维蛋白质印迹法检测了叶片和种子组织中ER同工酶的表达。在这两种组织中均表达了四种酶；种子材料中两种最丰富的同工型在叶片组织中也是最丰富的。
