Koller B, Gianfranceschi L, Seglias N, McDermott J, Gessler C
Institute of Plant Sciences, Swiss Federal Institute of Technology, ETH-Zürich.
Plant Mol Biol. 1994 Oct;26(2):597-602. doi: 10.1007/BF00013746.
Breeding resistant apple plants is an alternative way to control fungal pathogens reducing the environmental impact due to the use of pesticides. The breeding of apple cultivars resistant to Venturia inaequalis could be much improved by marker-assisted selection. A molecular marker closely linked to the resistance locus called Vf could replace selection based on infection studies. To find such molecular markers, DNA of progenies from crossings of a resistant and a susceptible apple tree was subject to bulked segregant analysis. Two markers were found with a genetic distance of 10.6% and 19.7% recombination frequency to the Vf locus.
培育抗性苹果植株是控制真菌病原体的一种替代方法，可减少因使用农药而对环境造成的影响。通过标记辅助选择，可以大大改进对苹果黑星病菌具有抗性的苹果品种的培育。与名为Vf的抗性基因座紧密连锁的分子标记可以取代基于感染研究的选择方法。为了找到这样的分子标记，对一棵抗性苹果树和一棵易感苹果树杂交后代的DNA进行了混合分离分析。发现了两个与Vf基因座的遗传距离分别为10.6%和19.7%重组频率的标记。
