Dipartimento di Colture Arboree, University of Bologna, 40126, Bologna, Italy.
Theor Appl Genet. 1996 May;92(7):803-10. doi: 10.1007/BF00221891.
Scab (Venturia inaequalis) is one of the most harmful diseases of apple, significantly affecting world apple production. The identification and early selection of resistant genotypes by molecular markers would greatly improve breeding strategies. Bulked segregant analysis was chosen for the identification of RAPD markers linked to the Vf scab resistant gene. Five different RAPD markers, derived from the wild species Malus floribunda. 821, were identified, and their genetic distance from Vf gene was estimated. The markers OPAM192200 and OPAL07580 were found to be very closely linked to the Vf gene. This result was indirectly confirmed by the analysis of resistant genotypes collected from various breeding programmes. Except for cv 'Murray', which carries the Vm gene, all these resistant genotypes showed the markers OPAM192200 and OPAL07580.
疮痂病（Venturia inaequalis）是苹果的一种主要病害，严重影响了世界苹果的产量。通过分子标记对具有抗性的基因型进行鉴定和早期选择，将极大地改进苹果的育种策略。本研究利用分离群体分组分析法（BSA）从野生种苹果绵蚜（Malus floribunda）821 中筛选与 Vf 抗疮痂病基因紧密连锁的 RAPD 标记。从苹果绵蚜 821 中筛选到 5 个与 Vf 基因连锁的 RAPD 标记，OPAM192200 和 OPAL07580 与 Vf 基因的遗传距离最近。来自不同育种计划的抗性基因型分析结果间接证实了这一结果。除了携带 Vm 基因的品种 Murray 外，所有这些抗性基因型都出现了 OPAM192200 和 OPAL07580 这两个标记。
