Boyen C, Leblanc C, Kloareg B, Loiseaux-de Goër S
Centre d'Etudes d'Océnologie et de Biologie Marine, CNRS-UPR 4601, Université P & M Curie, Roscoff, France.
Plant Mol Biol. 1994 Oct;26(2):691-7. doi: 10.1007/BF00013754.
Organellar DNA, i.e. a mixture of plastid and mitochondrial DNAs, was purified from the rhodophyte Chondrus crispus and analysed with restriction endonucleases. Mitochondrial DNA fragments were identified by heterologous hybridization, cloned, mapped and partially sequenced. The mitochondrial genome of C. crispus consists of a 25.9 kb circular molecule on which twenty genes were localized. Compared with other plant mitochondrial genomes, C. crispus mitochondrial DNA appears as a relatively small molecule with a high coding capacity and a specific gene organization. The use of a modified genetic code and the absence of RNA editing, previously reported for the cox3 gene, is a general characteristic of the sequenced genes of this molecule. This is the first detailed description of a red algal mitochondrial genome.
从红藻角叉菜中纯化出细胞器DNA，即质体DNA和线粒体DNA的混合物，并用限制性内切酶进行分析。通过异源杂交鉴定线粒体DNA片段，进行克隆、定位并部分测序。角叉菜的线粒体基因组由一个25.9 kb的环状分子组成，其上定位了20个基因。与其他植物线粒体基因组相比，角叉菜线粒体DNA呈现为一个相对较小的分子，具有高编码能力和特定的基因组织。先前报道的cox3基因使用修饰的遗传密码且不存在RNA编辑，是该分子测序基因的一个普遍特征。这是对红藻线粒体基因组的首次详细描述。
