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1
Mitochondrial genes in the colourless alga Prototheca wickerhamii resemble plant genes in their exons but fungal genes in their introns.无色藻类威克汉姆原囊藻中的线粒体基因,其外显子类似植物基因,但其内含子类似真菌基因。
Nucleic Acids Res. 1993 Feb 11;21(3):719-26. doi: 10.1093/nar/21.3.719.
2
Complete sequence of the mitochondrial DNA of the chlorophyte alga Prototheca wickerhamii. Gene content and genome organization.绿藻威克汉姆原藻线粒体DNA的完整序列。基因内容和基因组组织。
J Mol Biol. 1994 Mar 18;237(1):75-86. doi: 10.1006/jmbi.1994.1210.
3
Transcript mapping and processing of mitochondrial RNA in the chlorophyte alga Prototheca wickerhamii.绿藻威克汉姆原藻中线粒体RNA的转录本定位与加工
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4
The structural analysis of the mitochondrial SSUrRNA implies a close phylogenetic relationship between mitochondria from plants and from the heterotrophic alga Prototheca wickerhamii.线粒体小亚基核糖体RNA的结构分析表明,植物线粒体与异养藻类威克海姆原囊藻的线粒体之间存在密切的系统发育关系。
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6
The ribosomal RNA gene region in Acanthamoeba castellanii mitochondrial DNA. A case of evolutionary transfer of introns between mitochondria and plastids?卡氏棘阿米巴线粒体DNA中的核糖体RNA基因区域。线粒体与质体之间内含子发生进化转移的一个实例?
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A group-I intron in the mitochondrial large-subunit ribosomal RNA-encoding gene of Dictyostelium discoideum: same site localization in alga and in vitro self-splicing.盘基网柄菌线粒体大亚基核糖体RNA编码基因中的I组内含子:在藻类中的相同位点定位及体外自我剪接
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Fungal origin by horizontal transfer of a plant mitochondrial group I intron in the chimeric CoxI gene of Peperomia.真菌起源于胡椒属嵌合细胞色素氧化酶亚基I(CoxI)基因中植物线粒体I组内含子的水平转移。
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Molecular analysis of the split cox1 gene from the Basidiomycota Agrocybe aegerita: relationship of its introns with homologous Ascomycota introns and divergence levels from common ancestral copies.担子菌杨树菇分裂型细胞色素氧化酶亚基1基因的分子分析:其内含子与同源子囊菌内含子的关系及与共同祖先拷贝的分歧水平
Gene. 1998 Oct 5;220(1-2):45-53. doi: 10.1016/s0378-1119(98)00421-1.
10
Group I introns in the liverwort mitochondrial genome: the gene coding for subunit 1 of cytochrome oxidase shares five intron positions with its fungal counterparts.地钱线粒体基因组中的I组内含子:细胞色素氧化酶亚基1的编码基因与其真菌对应物共有五个内含子位置。
Nucleic Acids Res. 1993 Mar 11;21(5):1297-305. doi: 10.1093/nar/21.5.1297.

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A first insight into the genome of Prototheca wickerhamii, a major causative agent of human protothecosis.对人类原藻病主要病原体威克汉姆原藻基因组的初步洞察。
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The mitochondrial DNA of land plants: peculiarities in phylogenetic perspective.陆地植物的线粒体DNA:系统发育视角下的特性
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3
Complete sequence of the mitochondrial DNA of Chlamydomonas eugametos.衣藻线粒体DNA的完整序列。
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4
Genome structure and gene content in protist mitochondrial DNAs.原生生物线粒体DNA的基因组结构与基因含量
Nucleic Acids Res. 1998 Feb 15;26(4):865-78. doi: 10.1093/nar/26.4.865.
5
The reverse-transcriptase-like proteins encoded by group II introns in the mitochondrial genome of the brown alga Pylaiella littoralis belong to two different lineages which apparently coevolved with the group II ribosyme lineages.褐藻小海带线粒体基因组中II类内含子编码的逆转录酶样蛋白属于两个不同的谱系,它们显然与II类核酶谱系共同进化。
J Mol Evol. 1997 Jan;44(1):33-42. doi: 10.1007/pl00006119.
6
Transcript mapping and processing of mitochondrial RNA in the chlorophyte alga Prototheca wickerhamii.绿藻威克汉姆原藻中线粒体RNA的转录本定位与加工
Plant Mol Biol. 1996 Feb;30(3):577-95. doi: 10.1007/BF00049333.
7
Nucleotide sequence of the cox3 gene from Chondrus crispus: evidence that UGA encodes tryptophan and evolutionary implications.皱波角叉菜cox3基因的核苷酸序列:UGA编码色氨酸的证据及进化意义
Nucleic Acids Res. 1994 Apr 25;22(8):1400-3. doi: 10.1093/nar/22.8.1400.
8
Physical map and gene organization of the mitochondrial genome of Chondrus crispus (Rhodophyta, Gigartinales).皱波角叉菜(红藻门,杉藻目)线粒体基因组的物理图谱和基因组织
Plant Mol Biol. 1994 Oct;26(2):691-7. doi: 10.1007/BF00013754.
9
DNA sequence, structure, and phylogenetic relationship of the mitochondrial small-subunit rRNA from the red alga Chondrus crispus (Gigartinales rhodophytes).红藻角叉菜(杉藻目红藻)线粒体小亚基rRNA的DNA序列、结构及系统发育关系
J Mol Evol. 1995 Aug;41(2):196-202. doi: 10.1007/BF00170673.
10
Molecular phylogeny of Allomyces macrogynus: congruency between nuclear ribosomal RNA- and mitochondrial protein-based trees.大雌异水霉的分子系统发育:基于核糖体RNA和线粒体蛋白的树之间的一致性
J Mol Evol. 1995 Nov;41(5):657-65. doi: 10.1007/BF00175824.

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Chlamydomonas reinhardii gene for the 32 000 mol. wt. protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat.莱茵衣藻光合系统 II 32000 道尔顿蛋白的基因有四个大内含子,完全位于叶绿体反向重复区内。
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无色藻类威克汉姆原囊藻中的线粒体基因,其外显子类似植物基因,但其内含子类似真菌基因。

Mitochondrial genes in the colourless alga Prototheca wickerhamii resemble plant genes in their exons but fungal genes in their introns.

作者信息

Wolff G, Burger G, Lang B F, Kück U

机构信息

Lehrstuhl für Allgemeine Botanik, Ruhr-Universität Bochum, Germany.

出版信息

Nucleic Acids Res. 1993 Feb 11;21(3):719-26. doi: 10.1093/nar/21.3.719.

DOI:10.1093/nar/21.3.719
PMID:7680126
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309174/
Abstract

The mitochondrial DNA from the colourless alga Prototheca wickerhamii contains two mosaic genes as was revealed from complete sequencing of the circular extranuclear genome. The genes for the large subunit of the ribosomal RNA (LSUrRNA) as well as for subunit I of the cytochrome oxidase (coxI) carry two and three intronic sequences respectively. On the basis of their canonical nucleotide sequences they can be classified as group I introns. Phylogenetic comparisons of the coxI protein sequences allow us to conclude that the P.wickerhamii mtDNA is much closer related to higher plant mtDNAs than to those of the chlorophyte alga C.reinhardtii. The comparison of the intron sequences revealed several unusual features: (1) The P.wickerhamii introns are structurally related to mitochondrial introns from various ascomycetous fungi. (2) Phylogenetic analyses indicate a close relationship between fungal and algal intronic sequences. (3) The P. wickerhamii introns are located at positions within the structural genes which can be considered as preferred intron insertion sites in homologous mitochondrial genes from fungi or liverwort. In all cases, the sequences adjacent to the insertion sites are very well conserved over large evolutionary distances. Our finding of highly similar introns in fungi and algae is consistent with the idea that introns have already been present in the bacterial ancestors of present day mitochondria and evolved concomitantly with the organelles.

摘要

对无色藻类威克汉姆原囊藻的线粒体DNA进行环状核外基因组全序列测序后发现,其包含两个镶嵌基因。核糖体RNA大亚基(LSUrRNA)基因以及细胞色素氧化酶亚基I(coxI)基因分别携带两个和三个内含子序列。根据其典型核苷酸序列,它们可被归类为I组内含子。对coxI蛋白序列进行系统发育比较后,我们得出结论,威克汉姆原囊藻的线粒体DNA与高等植物的线粒体DNA的亲缘关系比与绿藻莱茵衣藻的线粒体DNA的亲缘关系更近。对内含子序列的比较揭示了几个不寻常的特征:(1)威克汉姆原囊藻的内含子在结构上与各种子囊菌的线粒体内含子相关。(2)系统发育分析表明真菌和藻类内含子序列之间存在密切关系。(3)威克汉姆原囊藻的内含子位于结构基因内的位置,这些位置可被视为真菌或地钱同源线粒体基因中优选的内含子插入位点。在所有情况下,插入位点附近的序列在很长的进化距离上都非常保守。我们在真菌和藻类中发现高度相似的内含子,这与内含子已存在于当今线粒体的细菌祖先中并与细胞器共同进化的观点一致。