Liu J S, Schardl C L
Department of Plant Pathology, University of Kentucky, Lexington 40546-0091.
Plant Mol Biol. 1994 Oct;26(2):775-8. doi: 10.1007/BF00013763.
A highly conserved sequence was found in rRNA gene internal transcribed spacer 1 (ITS1) among flowering plant species. The sequence, GGCRY-(4 to 7 n)-GYGYCAAGGAA (where Y = C or T; R = G or A) is located in the central region of ITS1, and is present in published sequences from a wide range of flowering plants. The rest of ITS1 is highly variable in sequence. Therefore, the conserved motif within ITS1 may have a key function in the processing of rRNA gene transcripts. Furthermore, identification of such a conserved motif will help facilitate alignment of sequences for phylogenetic analysis.
在开花植物物种的核糖体RNA基因内转录间隔区1(ITS1)中发现了一个高度保守的序列。该序列GGCRY-(4至7个核苷酸)-GYGYCAAGGAA(其中Y = C或T;R = G或A)位于ITS1的中央区域,并且存在于来自多种开花植物的已发表序列中。ITS1的其余部分在序列上高度可变。因此,ITS1内的保守基序可能在rRNA基因转录本的加工中具有关键功能。此外,鉴定这样一个保守基序将有助于促进用于系统发育分析的序列比对。