Orłowska-Majdak M, Goraca A, Głabiński A, Traczyk W Z
Department of Physiology, Medical University of Lodz, Poland.
J Physiol Pharmacol. 1994 Jun;45(2):309-17.
The aim of the experiments was an assessment of vasopressinergic (AVP-ergic) neurons response to somatic afferent impulsation in the spinal and in the cranial nerves. Experiments were carried out on male rats in urethane anaesthesia with the perfusion cannula introduced into the 3rd cerebral ventricle and with the catheter in the internal maxillary vein, in the vicinity of the cavernous sinus. Four samples of perfusing fluid, each of about 1.5 ml/30 min., and four samples of blood, 0.8 ml each, were collected. Vasopressin (AVP) was determined by radioimmunoassay in samples of perfusing fluid from the 3rd cerebral ventricle and in blood plasma. The central end of the left sciatic, supraorbital or infraorbital nerves was electrically stimulated with an intensity which increased the respiratory rate by 10-15% during collection of the IIIrd samples. The AVP concentration in the fluid perfusing the 3rd ventricle did not change during stimulation of the nerves. On the contrary, sciatic and supraorbital nerve stimulation caused a significant increase of AVP concentration in the blood. It can be concluded that sciatic and supraorbital nerve afferentation induced AVP release from the posterior pituitary lobe and did not affect AVP-ergic neurons projecting to the ventricular ependyma and responsible for AVP release into the cerebrospinal fluid.
实验的目的是评估血管加压素能（AVP能）神经元对脊髓和颅神经中躯体传入冲动的反应。实验在雄性大鼠身上进行，采用乌拉坦麻醉，将灌注插管插入第三脑室，并将导管置于海绵窦附近的上颌内静脉中。每30分钟收集约1.5毫升的四个灌注液样本和每个0.8毫升的四个血液样本。通过放射免疫分析法测定来自第三脑室的灌注液样本和血浆中的血管加压素（AVP）。在收集第三个样本期间，以能使呼吸频率增加10 - 15%的强度电刺激左侧坐骨神经、眶上神经或眶下神经的中枢端。在神经刺激期间，灌注第三脑室的液体中AVP浓度没有变化。相反，坐骨神经和眶上神经刺激导致血液中AVP浓度显著增加。可以得出结论，坐骨神经和眶上神经传入引起垂体后叶释放AVP，并且不影响投射到脑室室管膜并负责将AVP释放到脑脊液中的AVP能神经元。
