Lueders K K, Frankel W N
Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Mamm Genome. 1994 Aug;5(8):473-8. doi: 10.1007/BF00369315.
We present a linkage map of intracisternal A-particle (IAP) proviral loci. The IAP family consists of 2000 endogenous proviral elements that are widely dispersed in the mouse genome. The map was constructed by using an interspecific backcross and markers defined by oligonucleotide probes specific for subclasses of expressed IAP elements. In genomic DNA from C57BL/6J mouse, these probes each detected from 12 to 44 HindIII restriction fragments that represent junctions between proviral and 5'-flanking DNA. The fragments have characteristic strain distribution patterns (SDPs) that are particularly polymorphic in the DNAs of C57BL/6J and Mus spretus mice used for the backcross. IAP loci were placed on the map by comparison of their distribution patterns with those of known genetic markers in the backcross. The map includes 51 IAP loci that have not been previously mapped and 23 IAP proviruses that had been previously mapped in recombinant inbred (RI) strains. Comparable map positions were obtained with the IAP markers in the interspecific backcross and the RI strains. The mapped IAP loci were widely dispersed on the X Chromosome (Chr) and all of the autosomes except Chrs 9 and 19, providing useful genetic markers for linkage studies.
我们展示了脑内A颗粒（IAP）前病毒基因座的连锁图谱。IAP家族由2000个内源性前病毒元件组成，广泛分布于小鼠基因组中。该图谱是通过种间回交以及由针对表达的IAP元件亚类的寡核苷酸探针定义的标记构建而成。在C57BL/6J小鼠的基因组DNA中，这些探针各自检测到12至44个HindIII限制性片段，这些片段代表前病毒与5'侧翼DNA之间的连接。这些片段具有特征性的品系分布模式（SDPs），在用于回交的C57BL/6J和小家鼠（Mus spretus）小鼠的DNA中特别具有多态性。通过将IAP基因座与回交中已知遗传标记的分布模式进行比较，将其定位在图谱上。该图谱包括51个以前未定位的IAP基因座和23个以前在重组近交（RI）品系中定位的IAP前病毒。在种间回交和RI品系中，IAP标记获得了可比的图谱位置。定位的IAP基因座广泛分布在X染色体（Chr）以及除9号和19号染色体外的所有常染色体上，为连锁研究提供了有用的遗传标记。
