Deng Y Q
Dept. of TCM, 1st Affiliated Hospital, Zhejiang Medical Univ., Hangzhou.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1994 Mar;14(3):148-9, 132.
Serum adenosine deaminase (ADA) of 74 liver cirrhosis patients and 100 healthy subjects as control were examined with improved Martinek microassay and peripheral T lymphocyte subsets of 38 liver cirrhosis patients and 60 healthy subjects studied by indirect immunofluorescence assay (IFA) for exploring the relationship between them and syndrome types of TCM. The result showed that level of ADA of liver cirrhosis patients was higher than that of control (P < 0.01) and increased in following order: the type of Liver-energy Depression and Spleen Deficiency, that of Heat-Stagnation and Blood Stasis and that of Yin-Deficiency and Microvessel Obstruction. The difference of serum ADA among the types were significant (P < 0.01). The result also showed that OKT8 of liver cirrhosis patients was higher, the ratio of OKT4/OKT8 was lower than the healthy subjects (P < 0.05-0.01), but the difference among the types were not significant (P > 0.05). Serum ADA seemed to be one of the reference indexes in differentiating syndrome types of TCM, determining the patient's condition and prognosis.
采用改良的马丁内克微量分析法检测74例肝硬化患者及作为对照的100例健康受试者的血清腺苷脱氨酶（ADA），并采用间接免疫荧光法（IFA）研究38例肝硬化患者及60例健康受试者的外周血T淋巴细胞亚群，以探讨它们与中医证型之间的关系。结果显示，肝硬化患者的ADA水平高于对照组（P<0.01），且按肝郁脾虚型、热瘀互结型、阴虚络痹型依次升高。各证型间血清ADA差异有统计学意义（P<0.01）。结果还显示，肝硬化患者的OKT8升高，OKT4/OKT8比值低于健康受试者（P<0.05～0.01），但各证型间差异无统计学意义（P>0.05）。血清ADA似乎是中医辨证分型、判断病情及预后的参考指标之一。
