Otani Y, Okazaki I, Arai M, Kameyama K, Wada N, Maruyama K, Yoshino K, Kitajima M, Hosoda Y, Tsuchiya M
Department of Surgery, School of Medicine, Keio University, Tokyo, Japan.
J Gastroenterol. 1994 Aug;29(4):391-7. doi: 10.1007/BF02361233.
Collagenolytic proteinases play an important pathological role in the invasion and metastasis of cancer cells. However, little is known about the role of interstitial collagenase (matrix metalloproteinase-1; MMP-1) in this process. To investigate the expression of the MMP-1 gene in cancer tissues, an in situ hybridization study was carried out in gastrointestinal tract cancers (one esophageal cancer, five gastric cancers, and four colorectal cancers), using a 35S-labeled MMP-1 cDNA probe. The MMP-1 gene was expressed in the stromal cells of fibrous tissue around cancer nests, especially at the margin of invasion and/or within the cancer nest; however, no definite expression within cancer cells was observed. Expression of the MMP-1 gene in the stromal cells was more common in well differentiated gastric adenocarcinoma than in poorly differentiated adenocarcinomas. These findings indicate that expression of the MMP-1 gene is greater in stromal cells that are closely associated with cancer cells, suggesting a pathophysiological role of MMP-1 in the invasion and metastasis of cancer cells.
胶原溶解蛋白酶在癌细胞的侵袭和转移过程中发挥着重要的病理作用。然而，关于间质胶原酶（基质金属蛋白酶-1；MMP-1）在此过程中的作用却知之甚少。为了研究MMP-1基因在癌组织中的表达情况，我们使用35S标记的MMP-1 cDNA探针，对胃肠道癌症（1例食管癌、5例胃癌和4例结直肠癌）进行了原位杂交研究。MMP-1基因在癌巢周围纤维组织的基质细胞中表达，尤其是在侵袭边缘和/或癌巢内；然而，未观察到癌细胞内有明确表达。MMP-1基因在基质细胞中的表达在高分化胃腺癌中比低分化腺癌中更常见。这些发现表明，与癌细胞密切相关的基质细胞中MMP-1基因的表达更高，提示MMP-1在癌细胞的侵袭和转移中具有病理生理作用。
