Aida Y, Maeyama S, Takakuwa T, Uchikoshi T, Endo Y, Suzuki K, Taniguchi N
Second Department of Pathology, St. Marianna University School of Medicine, Kanagawa, Japan.
J Gastroenterol. 1994 Aug;29(4):443-9. doi: 10.1007/BF02361241.
The expression of manganese superoxide dismutase (Mn-SOD) was studied immunohistochemically, using a specific monoclonal antibody, in surgically resected hepatocellular carcinoma (HCC) and noncancerous tissues from 47 patients (2 with well-differentiated HCC, 36 with moderately differentiated HCC, 8 with poorly differentiated HCC, and 1 with undifferentiated carcinoma). Cancer cells in 44 patients (93.6%) were positive for Mn-SOD. The staining pattern of cancer cells was mostly homogeneous in well-differentiated HCC, whereas it was heterogeneous in poorly differentiated HCC. Moreover, strongly positive immunoreactivity was observed in noncancerous liver tissues in all patients, especially in normal hepatocytes surrounding HCC, regenerative small hepatocytes in the tumor boundary, and mononuclear inflammatory cells in the necroinflammatory lesions. The positive immunoreactivity for Mn-SOD in patients with HCC appears to reflect increased production of the enzyme protein.
利用一种特异性单克隆抗体，通过免疫组织化学方法研究了47例患者手术切除的肝细胞癌（HCC）及癌旁组织中锰超氧化物歧化酶（Mn-SOD）的表达情况（2例高分化HCC、36例中分化HCC、8例低分化HCC和1例未分化癌）。44例患者（93.6%）的癌细胞Mn-SOD呈阳性。高分化HCC中癌细胞的染色模式大多均匀一致，而低分化HCC中则呈异质性。此外，所有患者的癌旁肝组织均观察到强阳性免疫反应，尤其是HCC周围的正常肝细胞、肿瘤边界处的再生小肝细胞以及坏死性炎症病变中的单核炎性细胞。HCC患者中Mn-SOD的阳性免疫反应似乎反映了该酶蛋白产生的增加。
