Iizuka S, Taniguchi N, Makita A
J Natl Cancer Inst. 1984 May;72(5):1043-9.
A manganese-containing superoxide dismutase (Mn-SOD) was purified from human liver. Polyclonal antibody for the Mn-SOD was prepared in goat, and a simple and specific enzyme-linked immunosorbent assay (ELISA) for the Mn-SOD was developed. This assay was found to be sensitive to nanogram amounts of the enzyme. With respect to Mn-SOD levels of normal lung tissues, a positive correlation (r = 0.92, P less than .001) was observed between the results of enzymatic assay and those of immunochemical assay by ELISA. In lung carcinoma tissue the enzyme activity was in the same order of magnitude as in uninvolved tissues. However, the enzyme content determined by ELISA was significantly higher in adenocarcinoma than in the uninvolved lung tissue, whereas no significant difference from the control was observed in other histologic types. The discrepancy between the enzyme activity and immunoreactive content suggested that in the adenocarcinoma of the lung an immunoreactive but enzymatically inactive Mn-SOD protein existed and that a high content of this enzyme was characteristic of lung adenocarcinoma.
从人肝脏中纯化出一种含锰超氧化物歧化酶（Mn-SOD）。用山羊制备了针对Mn-SOD的多克隆抗体，并开发了一种简单且特异的Mn-SOD酶联免疫吸附测定法（ELISA）。发现该测定法对纳克量的该酶敏感。关于正常肺组织的Mn-SOD水平，酶法测定结果与ELISA免疫化学测定结果之间观察到正相关（r = 0.92，P <.001）。在肺癌组织中，酶活性与未受累组织处于相同数量级。然而，ELISA测定的酶含量在腺癌中明显高于未受累的肺组织，而在其他组织学类型中与对照无显著差异。酶活性与免疫反应性含量之间的差异表明，在肺腺癌中存在一种具有免疫反应性但无酶活性的Mn-SOD蛋白，且该酶的高含量是肺腺癌的特征。
