高效液相色谱法测定人胎儿肾上腺中的去甲肾上腺素、肾上腺素和多巴胺。

High-performance liquid chromatographic determination of norepinephrine, epinephrine and dopamine in human foetal adrenal gland.

作者信息

Garciá J C, Blanco L, McPherson M, Leiva A, Maciás R

机构信息

Centro Internacional de Restauracion Neurologica, Habana, Cuba.

出版信息

J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):77-80. doi: 10.1016/0378-4347(94)00088-3.

DOI:10.1016/0378-4347(94)00088-3

PMID:7952050

Abstract

The purpose of this work was to characterize the human foetal adrenal gland (HFAG) by studying norepinephrine (NE), epinephrine (E), and dopamine (DA) levels in foetuses with ages ranging from 10 to 18 weeks, as a source of chromaffin cells for basic and clinical research, in the treatment of Parkinson's disease. The HFAGs were obtained from ten abortions with foetal ages ranging from 10-12 (n = 7) to 13-18 (n = 3) weeks. For the simultaneous detection of NE, E, and DA a high-performance liquid chromatographic (HPLC) procedure with electrochemical detection was employed. The concentrations found (ng/mg wet weight) were 24.24 +/- 7.06 for NE, 3.56 +/- 2.97 for E and 0.45 +/- 0.27 for DA at 10-12 weeks and 14.91 +/- 9.94 for NE, 8.18 +/- 8.79 for E and 0.16 +/- 0.05 for DA at 13-18 weeks. The DA:E ratio present in HFAG between 10 and 12 weeks was 100 times higher than that reported by other authors in adult adrenal medulla.

摘要

这项工作的目的是通过研究10至18周龄胎儿体内的去甲肾上腺素（NE）、肾上腺素（E）和多巴胺（DA）水平来表征人类胎儿肾上腺（HFAG），将其作为嗜铬细胞的来源用于基础和临床研究以及帕金森病的治疗。HFAG取自10例堕胎胎儿，胎龄范围为10 - 12周（n = 7）至13 - 18周（n = 3）。为同时检测NE、E和DA，采用了带有电化学检测的高效液相色谱（HPLC）方法。在10 - 12周时，所测得的浓度（ng/毫克湿重）分别为：NE为24.24±7.06，E为3.56±2.97，DA为0.45±0.27；在13 - 18周时，NE为14.91±9.94，E为8.18±8.79，DA为0.16±0.05。10至12周龄的HFAG中DA与E的比值比其他作者报道的成人肾上腺髓质中的比值高100倍。