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高效液相色谱结合电化学检测作为研究儿茶酚胺能系统的高效工具。I. 培养的肾上腺髓质细胞中去甲肾上腺素、肾上腺素和多巴胺的定量分析。

High-performance liquid chromatography with electrochemical detection as a highly efficient tool for studying catecholaminergic systems. I. Quantification of noradrenaline, adrenaline and dopamine in cultured adrenal medullary cells.

作者信息

Müller T H, Unsicker K

出版信息

J Neurosci Methods. 1981 Jun;4(1):39-52. doi: 10.1016/0165-0270(81)90017-0.

DOI:10.1016/0165-0270(81)90017-0
PMID:7253699
Abstract

A method for simultaneous determinations of noradrenaline (NA), adrenaline (A) and dopamine (DA) in tissue is described. After extraction from crude deproteinized homogenates by the batch mode aluminium oxide method individual catecholamines (CA) were separated in a high-performance liquid chromatography (HPLC) system by isocratic elution with pure aqueous buffers from an octadecyl column. These eluates were directly monitored with a new amperometric detector device (Metrohm VA E 641) in a wall-jet flow cell. Sensitivity, specificity, precision and performance of this relatively simple procedure are characterized. Different applications of this assay with relevance to neurobiological research are presented.

摘要

描述了一种同时测定组织中去甲肾上腺素（NA）、肾上腺素（A）和多巴胺（DA）的方法。通过分批模式氧化铝法从粗制脱蛋白匀浆中提取后，采用高效液相色谱（HPLC）系统，使用纯水缓冲液等度洗脱，从十八烷基柱上分离出各个儿茶酚胺（CA）。这些洗脱液在壁流池中的新型安培检测器装置（万通VA E 641）上直接进行监测。对这个相对简单的程序的灵敏度、特异性、精密度和性能进行了表征。介绍了该测定法在神经生物学研究中的不同应用。

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