Whitty P W, Powell W, Sprent J I
Department of Biological Sciences, University of Dundee, UK.
Mol Ecol. 1994 Oct;3(5):507-15. doi: 10.1111/j.1365-294x.1994.tb00129.x.
Randomly Amplified Polymorphic DNA (RAPD) methods have been adapted for use as a phenetic tool on the legume tribe Cassiinae. RAPD-generated polymorphism within local populations was lower than between populations from different geographic regions, between species and genera. Examination of three Cassia species, 12 Chamaecrista species and 13 Senna species using eight primers showed the potential for separation of the nodulated/nitrogen fixing genus Chamaecrista from the previously congeneric groups Cassia and Senna. Similarly, RAPD analysis of two groups of nine Ch. rotundifolia and nine Ch. mimosoides samples using 11 primers has given separation according to both species and to geographical location. Analysis of a small sample of five Chamaecrista species from Brazil with eight primers gave separation consistent with known variations in nodule structure.
随机扩增多态性DNA（RAPD）方法已被改编用作豆科决明亚族的一种表型分析工具。当地种群内由RAPD产生的多态性低于不同地理区域的种群之间、物种之间和属之间的多态性。使用8种引物对3种决明属植物、12种决明草属植物和13种番泻属植物进行检测，结果表明有可能将结瘤/固氮的决明草属与之前同属的决明属和番泻属区分开来。同样，使用11种引物对两组各9个圆叶决明样本和9个含羞草决明样本进行RAPD分析，结果根据物种和地理位置都实现了区分。用8种引物对来自巴西的5种决明草属植物的小样本进行分析，得到的区分结果与已知的根瘤结构变异一致。
