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RAPD技术在罗非鱼中的应用:物种和亚种鉴定。

Application of the RAPD technique in tilapia fish: species and subspecies identification.

作者信息

Bardakci F, Skibinski D O

机构信息

Molecular Biology Research Group, School of Biological Sciences, University of Wales, Swansea, U.K.

出版信息

Heredity (Edinb). 1994 Aug;73 ( Pt 2):117-23. doi: 10.1038/hdy.1994.110.

DOI:10.1038/hdy.1994.110
PMID:8071079
Abstract

Random Amplified Polymorphic DNA (RAPD) analysis was applied to three species of the tilapia genus Oreochromis and four subspecies of O. niloticus. Thirteen random 10-mer primers were used to assay polymorphisms within and between populations. Different RAPD fragment patterns were observed for different species, although not always for different subspecies. Evidence is presented that RAPD markers might be useful for systemic investigation at the level of species and subspecies.

摘要

随机扩增多态性DNA(RAPD)分析被应用于罗非鱼属的三个物种和尼罗罗非鱼的四个亚种。使用13个随机的10聚体引物来检测群体内部和群体之间的多态性。不同物种观察到不同的RAPD片段模式,尽管不同亚种并非总是如此。有证据表明,RAPD标记可能有助于在物种和亚种水平上进行系统研究。

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