对输入性恶性疟原虫疟疾的一种新型聚合酶链反应靶点（STEVOR）的前瞻性评估。

Prospective assessment of a new polymerase chain reaction target (STEVOR) for imported Plasmodium falciparum malaria.

作者信息

Filisetti D, Bombard S, N'Guiri C, Dahan R, Molet B, Abou-Bacar A, Hansmann Y, Christmann D, Candolfi E

机构信息

Institut de Parasitologie et de Pathologie Tropicale, 3 rue Koeberlé, 67000 Strasbourg, France.

出版信息

Eur J Clin Microbiol Infect Dis. 2002 Sep;21(9):679-81. doi: 10.1007/s10096-002-0800-7. Epub 2002 Sep 6.

DOI:10.1007/s10096-002-0800-7

PMID:12373502

Abstract

The diagnostic value of a polymerase chain reaction (PCR)-based method for amplifying a new target of repeated genes (STEVOR) in Plasmodium falciparum was prospectively assessed on samples from 210 febrile patients returning from areas endemic for malaria. This method is capable of detecting 0.01 parasites in one microliter of blood. Plasmodium falciparum STEVOR PCR confirmed the results of the thin- and thick-film direct examination method but identified Plasmodium falciparum in four patients in whom direct examination was inconclusive at the species level. Moreover, PCR was positive in two patients with a negative direct examination. Thus, Plasmodium falciparum STEVOR PCR had 100% sensitivity and specificity and could be used in selected parasitology laboratories when expert advice is required.

摘要

前瞻性评估了一种基于聚合酶链反应（PCR）的方法在扩增恶性疟原虫重复基因新靶点（STEVOR）方面的诊断价值，该评估针对的是210名从疟疾流行地区返回的发热患者的样本。这种方法能够在一微升血液中检测出0.01个疟原虫。恶性疟原虫STEVOR PCR证实了薄血膜和厚血膜直接检查方法的结果，但在4名直接检查在物种水平上结果不明确的患者中鉴定出了恶性疟原虫。此外，在两名直接检查为阴性的患者中PCR呈阳性。因此，恶性疟原虫STEVOR PCR具有100%的敏感性和特异性，在需要专家建议时可用于特定的寄生虫学实验室。