通过对克隆于大肠杆菌中的海胆（紫球海胆）DNA进行序列分析来鉴定组蛋白H2A和H3基因及其位置。

Identification and location of the histone H2A and H3 genes by sequence analysis of sea urchin (S. purpuratus) DNA cloned in E. coli.

作者信息

Sures I, Maxam A, Cohn R H, Kedes L H

出版信息

Cell. 1976 Dec;9(4 Pt 1):495-502. doi: 10.1016/0092-8674(76)90031-3.

Abstract

A 2000 base pair (bp) DNA fragment can be excised from sea urchin (S. purpuratus) histone gene repeat units with restriction endonuclease Eco R1. This DNA, which has been cloned in a bacterial plasmid, is known to encompass two of the five histone genes. The fragment has a single endonuclease Hind III cleavage site in one of the genes and a Hae III cleavage site in the other gene. We now report the nucleotide sequences of 62 bp adjacent to the Hind III site and 42 bp adjacent to the Hae III cleavage site. The results identify the cloned DNA as histone genes, show that it codes for histone proteins H2A and H3, and locate and orient H2A and H3 genes with respect to restriction endonuclease sites in the repeat unit.

摘要

用限制性内切酶Eco R1可从海胆（紫球海胆）组蛋白基因重复单元中切下一段2000个碱基对（bp）的DNA片段。这段已克隆到细菌质粒中的DNA已知包含五个组蛋白基因中的两个。该片段在其中一个基因中有一个单一的内切酶Hind III切割位点，在另一个基因中有一个Hae III切割位点。我们现在报告与Hind III位点相邻的62 bp和与Hae III切割位点相邻的42 bp的核苷酸序列。结果确定克隆的DNA为组蛋白基因，表明它编码组蛋白H2A和H3，并相对于重复单元中的限制性内切酶位点定位和确定H2A和H3基因的方向。