Sures I, Levy S, Kedes L H
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1265-9. doi: 10.1073/pnas.77.3.1265.
We have determined the sequence of the untranslated leader nucleotides of all five histone mRNAs from Strongylocentrotus purpuratus by the dideoxy chain termination method. Total polysomal RNA from sea urchin embryos was used as a substrate for cDNA synthesis primed by specific DNA restriction fragments. Each of the primers was derived from the 5'-terminal part of the coding region for a different histone protein. The five histone mRNA leader sequences are different in length and primary structure. The 5' termini of all five histone mRNAs coincide with the unique heptanucleotide Py-Py-A-T-T-C-Pu in genomic DNA. This sequence, which defines the start of the individual histone mRNAs, is preceded by the A+T-rich octanucleotide identified in front of all eukaryotic structural genes where sequences have been determined to date.
我们采用双脱氧链终止法测定了紫球海胆所有五种组蛋白mRNA的非翻译前导核苷酸序列。来自海胆胚胎的总多聚核糖体RNA用作由特定DNA限制片段引发的cDNA合成的底物。每个引物均来自不同组蛋白编码区的5'末端部分。这五个组蛋白mRNA前导序列在长度和一级结构上有所不同。所有五种组蛋白mRNA的5'末端均与基因组DNA中独特的七核苷酸Py-Py-A-T-T-C-Pu一致。该序列定义了各个组蛋白mRNA的起始位置，在其之前是在所有已确定序列的真核结构基因前发现的富含A+T的八核苷酸。
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