Sheridan B L, Lollo M, Howe S, Bergeron N
Department of Laboratory Haematology, Sunnybrook Health Science Centre, Toronto, Ontario, Canada.
Clin Lab Haematol. 1994 Jun;16(2):117-30. doi: 10.1111/j.1365-2257.1994.tb00398.x.
The performance of the Roche Cobas Argos 5Diff (Argos) automated haematology analyser was evaluated by comparison to manual blood film examination and a Coulter STKS (STKS) analyser. The Argos demonstrated excellent between and inter-batch imprecision for all parameters, except the MCHC, and good linearity for Hb, WBC and platelet count (PLT). After an initial fall the PLT, results were stable for up to six h at 18 degrees C in EDTA(K3) after which an increasing proportion of cells were classified as lymphocytes. Results of 239 patient samples analysed on both instruments, compared by linear regression, gave excellent correlation (r2 > 0.90) for most parameters with the exceptions of the MCHC (0.317), eosinophils% (0.756), monocytes% (0.48) and basophils% (0.002). 'Flagging' of cellular abnormalities by the Argos resulted in excellent sensitivity (97.5%), specificity (93.2%) and efficiency/agreement (93.2%), with fewer false positive and negative results than the STKS, although these differences were not statistically significant. The performance characteristics of the Argos were comparable to those of the STKS with a possible improvement in its flagging abilities.
通过与手工血涂片检查和库尔特STKS分析仪进行比较，对罗氏Cobas Argos 5Diff（Argos）全自动血液分析仪的性能进行了评估。除平均红细胞血红蛋白浓度（MCHC）外，Argos在所有参数方面均表现出出色的批间和批内不精密度，并且在血红蛋白（Hb）、白细胞（WBC）和血小板计数（PLT）方面具有良好的线性。血小板计数在初始下降后，在18摄氏度的乙二胺四乙酸（K3）中可稳定长达6小时，之后越来越多的细胞被分类为淋巴细胞。对239份患者样本在两台仪器上进行分析，通过线性回归比较，除MCHC（0.317）、嗜酸性粒细胞百分比（0.756）、单核细胞百分比（0.48）和嗜碱性粒细胞百分比（0.002）外，大多数参数具有极好的相关性（r2>0.90）。Argos对细胞异常的“标记”具有出色的敏感性（97.5%）、特异性（93.2%）和效率/一致性（93.2%），假阳性和假阴性结果比STKS少，尽管这些差异无统计学意义。Argos的性能特征与STKS相当，其标记能力可能有所改进。
