al-Ismail S A, Bond K, Carter A B, Grant D, Machin S J, Patterson K G, Pearman K J, Pollard Y C, Popeck M
Haematology Department, Morriston Hospital, Swansea, Wales, UK.
Clin Lab Haematol. 1995 Mar;17(1):11-21. doi: 10.1111/j.1365-2257.1995.tb00311.x.
The CD3500 blood counter (Abbott Laboratories) is a 33 parameter fully automated blood counter that produces a five part differential count with flagging of leucocyte abnormalities. In this evaluation excellent correlation between CD3500 and Coulter STKR blood counter was found for all red cell and platelet parameters on the 221 samples tested. Studies of carryover, mixing efficiency and precision also gave excellent results. There was a good correlation with manual 400 cell differential counts for neutrophils, lymphocytes, monocytes and eosinophils for the 468 samples compared. Correlation of CD3500 and manual basophil counts was poor. Normal samples stored at 4 degrees C and analysed while cold showed satisfactory stability for WBC, RBC, Hb, MCV and platelets for 48 h and a stable differential for 24 h. Correlation with the differential count produced by the Coulter STKS showed good correlation for neutrophils, lymphocytes, monocytes and eosinophils; correlation with STKS basophils was poor. False positive flagging rate varied between 8.9% (Band and/or IG) and 0.9% (NRBC) depending on the nature of the flag; 5.8% of samples exhibited two or more false positive flags. No significant breakdowns were encountered during the period of the evaluation. The scatterplot displays of laser light scatter produced by the instrument provide an interesting adjunct to conventional morphology.
CD3500血液计数器（雅培实验室）是一款具有33项参数的全自动血液计数器，可进行五分类白细胞计数并标记白细胞异常情况。在本次评估中，对221份检测样本的所有红细胞和血小板参数进行检测，发现CD3500与库尔特STKR血液计数器之间具有极佳的相关性。对携带污染、混合效率和精密度的研究也取得了优异的结果。在对比的468份样本中，中性粒细胞、淋巴细胞、单核细胞和嗜酸性粒细胞的CD3500计数与手工400细胞分类计数具有良好的相关性。CD3500与手工嗜碱性粒细胞计数的相关性较差。储存在4℃并在低温状态下进行分析的正常样本，其白细胞、红细胞、血红蛋白、平均红细胞体积和血小板在48小时内显示出令人满意的稳定性，分类计数在24小时内保持稳定。与库尔特STKS产生的分类计数相比，中性粒细胞、淋巴细胞、单核细胞和嗜酸性粒细胞具有良好的相关性；与STKS嗜碱性粒细胞的相关性较差。假阳性标记率根据标记的性质在8.9%（杆状核和/或幼稚粒细胞）至0.9%（有核红细胞）之间变化；5.8%的样本出现两个或更多假阳性标记。在评估期间未遇到重大故障。该仪器产生的激光散射散点图为传统形态学提供了有趣的辅助手段。
