Salazar G, Infante C, Vio F
Institute of Nutrition and Food Technology, University of Chile, Santiago.
Eur J Clin Nutr. 1994 Jul;48(7):475-81.
Equilibration time of deuterium oxide was studied in infants with no restriction of food intake, as in field work stringent conditions have to be relaxed.
Ten children recovering from marasmatic undernutrition in an Infant Nutrition Centre (CONIN).
Four males and six females [mean 253 +/- 38 (SD) days old] exclusively bottle-fed were administered a deuterium dose in their first morning feed and followed for a 12 day period. The intercept method was used as the reference to validate the isotope equilibration time within the body water pool calculated with the plateau method. Mean equilibration was found to be 291 +/- 78 (SD) (4.85 +/- 1.3 h).
Total body water (TBW) values calculated with both methods differed only by 0.6%, confirming that the deuterium oxide was homogeneously distributed within the body water pool at the plateau equilibration time (4.85 +/- 1.3 h). This period is much larger than the one commonly utilized, 120-180 min (2-3 h).
Regarding TBW measurements, the intercept method provides the same information as the plateau method. However, the intercept method seems more suitable for field studies when working conditions cannot be controlled. The plateau method can be useful for specific short studies providing a faster assessment of the body water pool in infants.
研究在食物摄入无限制的婴儿中氧化氘的平衡时间，因为在实地工作中必须放宽严格的条件。
在一家婴儿营养中心（CONIN）从消瘦型营养不良中恢复的10名儿童。
4名男性和6名女性[平均年龄253±38（标准差）天]，均为人工喂养，在第一天早晨喂奶时给予氘剂量，并跟踪12天。采用截距法作为参考，以验证用平台法计算的体内水池中同位素平衡时间。发现平均平衡时间为291±78（标准差）（4.85±1.3小时）。
两种方法计算的全身水（TBW）值仅相差0.6%，证实氧化氘在平台平衡时间（4.85±1.3小时）时在体内水池中均匀分布。这个时间段比常用的120 - 180分钟（2 - 3小时）长得多。
关于TBW测量，截距法与平台法提供相同的信息。然而，当工作条件无法控制时，截距法似乎更适合实地研究。平台法对于特定的短期研究可能有用，能更快地评估婴儿的体内水池。
