Wirsing von König C H, Finger H
Institut für Hygiene und Laboratoriumsmedizin, Städtische Krankenanstalten, Krefeld, Germany.
Eur J Clin Microbiol Infect Dis. 1994 Jun;13(6):455-8. doi: 10.1007/BF01974634.
Whooping cough can be caused by either Bordetella pertussis or Bordetella parapertussis. Although the two species share an almost complete DNA identity, Bordetella parapertussis does not produce pertussis toxin, which is thought to be the main virulence factor of Bordetella pertussis. In order to elucidate the role of pertussis toxin in causing the typical symptoms of whooping cough, clinical information from 33 patients with culture-positive Bordetella parapertussis infection was collected and compared to that from 331 patients with infection caused by Bordetella pertussis. Isolated strains of Bordetella parapertussis lacked pertussis toxin expression, as was demonstrated by negative tests for histamine sensitization. This was further substantiated in vivo by a significantly lower leukocyte count in the parapertussis group as compared to the pertussis group. Frequencies of typical symptoms of whooping cough, such as paroxysmal coughing, whooping and vomiting, were almost identical in the two groups. Nocturnal coughing and contact anamnesis were noted more often in the Bordetella pertussis group. Children in the parapertussis group were significantly more often vaccinated with whole-cell pertussis vaccine than children infected with Bordetella pertussis. The results indicate that pertussis toxin may not play a decisive role in causing the typical symptoms of whooping cough, such as paroxysmal coughing, whooping and vomiting.
百日咳可由百日咳博德特氏菌或副百日咳博德特氏菌引起。尽管这两个菌种几乎具有完全相同的DNA，但副百日咳博德特氏菌不产生百日咳毒素，而百日咳毒素被认为是百日咳博德特氏菌的主要毒力因子。为了阐明百日咳毒素在引发百日咳典型症状中的作用，收集了33例副百日咳博德特氏菌培养阳性感染患者的临床信息，并与331例百日咳博德特氏菌感染患者的临床信息进行比较。副百日咳博德特氏菌的分离菌株缺乏百日咳毒素表达，组胺致敏试验呈阴性证明了这一点。与百日咳组相比，副百日咳组白细胞计数显著降低，这在体内进一步证实了上述结果。两组百日咳典型症状（如阵发性咳嗽、哮吼和呕吐）的发生率几乎相同。百日咳博德特氏菌组夜间咳嗽和接触史更为常见。副百日咳组儿童接种全细胞百日咳疫苗的频率显著高于百日咳博德特氏菌感染儿童。结果表明，百日咳毒素在引发百日咳典型症状（如阵发性咳嗽、哮吼和呕吐）中可能不起决定性作用。
