Holt W, Watson P, Curry M, Holt C
Institute of Zoology, Zoological Society of London, United Kingdom.
Fertil Steril. 1994 Dec;62(6):1277-82.
To assess a single (donor) sample by the use of five computer-assisted semen analysis (CASA) systems.
British Andrology Society advanced course on computer-assisted semen analysis techniques, September 1992.
Clinical, technical and scientific personnel with mixed experience of CASA techniques, but all interested in semen assessment technology.
22 sets of data, comprising 6 commonly derived parameters, from the same sample were obtained using five CASA systems. The coefficients of variation for sperm concentration (29%) and proportion motile (24%) were comparable to those previously reported for manual assessments. For all parameters, "within system" variability was considerably greater than "between system," indicating that differences in sample handling and operator expertise were more significant sources of variation than the CASA systems themselves.
Emphasis on operator training and standardization of sample handling techniques would enhance the reproducibility of CASA measurements more than improvements in the CASA systems themselves. There was evidence, however, that the CASA measurements were more consistent than equivalent manual measures, and also provide information about the quality of sperm motility which cannot be obtained by alternative techniques.
使用五种计算机辅助精液分析（CASA）系统对单个（供体）样本进行评估。
1992年9月，英国男科学会计算机辅助精液分析技术高级课程。
具备CASA技术混合经验，但均对精液评估技术感兴趣的临床、技术和科研人员。
使用五种CASA系统从同一样本中获取了22组数据，包括6个常用衍生参数。精子浓度（29%）和活动比例（24%）的变异系数与之前手动评估报告的结果相当。对于所有参数，“系统内”变异性远大于“系统间”，这表明样本处理差异和操作人员专业水平是比CASA系统本身更显著的变异来源。
相较于改进CASA系统本身，强调操作人员培训和样本处理技术标准化将更有助于提高CASA测量的可重复性。然而，有证据表明，CASA测量比同等的手动测量更一致，并且还能提供替代技术无法获得的精子活力质量信息。
