Matern H T, Klein J R, Henrich B, Plapp R
Universität Kaiserslautern, Fachbereich Biologie, Abteilung Mikrobiologie, FRG.
FEMS Microbiol Lett. 1994 Sep 15;122(1-2):121-8. doi: 10.1111/j.1574-6968.1994.tb07154.x.
The transcriptional start points of ten Lactobacillus delbrückii ssp. lactis DSM7290 genes were determined by primer extension. The upstream located promoter regions, including potential -35 and -10 regions and the spacing between them were compared to the well-known Escherichia coli and Bacillus subtilis promoters. The Lb. delbrückii -35 consensus sequence (TTGACA) seems to be less conserved then the E. coli sequence. The nucleotides TGC were often found upstream of the -10 region (TATAAT). The most frequently observed spacing between the two core promoter regions was 17 nt and the main distance between the -10 region and the transcriptional start point was mostly determined to be 6 nt in contrast to 7 nt, as described for E. coli promoters. The preferred initiation nucleotides in Lb. delbrückii were shown to be definitely purines (A or G). The ribosome binding sites located downstream of the promoters revealed the consensus sequence 3'-UCCUCCU-5', being the predicted 3'-OH end of the Lactobacillus 16S rRNA with a high degree of homology to known 16S rRNAs.
通过引物延伸法确定了德氏乳杆菌乳酸亚种DSM7290的10个基因的转录起始点。将位于上游的启动子区域，包括潜在的-35区和-10区以及它们之间的间隔，与著名的大肠杆菌和枯草芽孢杆菌启动子进行了比较。德氏乳杆菌的-35共有序列（TTGACA）似乎不如大肠杆菌序列保守。在-10区（TATAAT）上游经常发现核苷酸TGC。两个核心启动子区域之间最常观察到的间隔为17个核苷酸，-10区与转录起始点之间的主要距离大多确定为6个核苷酸，而大肠杆菌启动子的这一距离为7个核苷酸。德氏乳杆菌中优选的起始核苷酸被证明绝对是嘌呤（A或G）。位于启动子下游的核糖体结合位点显示共有序列为3'-UCCUCCU-5'，这是乳酸杆菌16S rRNA预测的3'-OH末端，与已知的16S rRNAs具有高度同源性。
