Dirks W, Schaper F, Kirchhoff S, Morelle C, Hauser H
Department of Human and Animal Cell Culture, DSM--German Collection of Microorganisms and Cell Cultures, Braunschweig.
Gene. 1994 Nov 18;149(2):387-8. doi: 10.1016/0378-1119(94)90186-4.
A family of different promoters and vector backbones for multiple use in mammalian cells in vivo and in vitro experiments has been created. The elements allow high-level constitutive, as well as adjustable expression with mono- and dicistronic transcription units and several in vitro manipulations of the inserted ORFs. The vector components are designed to be combined in a simple cloning step.