Dirks W, Wirth M, Hauser H
Eukaryotic Genetics, GBF-Gesellschaft für Biotechnologische Forschung mbH, Braunschweig, Germany.
Gene. 1993 Jun 30;128(2):247-9. doi: 10.1016/0378-1119(93)90569-o.
Dicistronic vectors utilizing the internal ribosomal entry site sequence of poliovirus as the intercistronic region were constructed for gene expression in mammalian cells. We have developed two monocistronic expression vectors which facilitate the creation of dicistronic expression plasmids. The dicistronic expression plasmids encode transcription units which allow the coordinated translation of the two genes. Using internal luciferase and secreted alkaline phosphatase, we show the correlated expression of both reporter genes and expression levels comparable to those achieved by the respective monocistronic expression vectors.
构建了利用脊髓灰质炎病毒内部核糖体进入位点序列作为顺反子间区域的双顺反子载体，用于在哺乳动物细胞中进行基因表达。我们开发了两种单顺反子表达载体，便于构建双顺反子表达质粒。双顺反子表达质粒编码转录单元，可实现两个基因的协调翻译。利用内部荧光素酶和分泌型碱性磷酸酶，我们展示了两个报告基因的相关表达以及与各自单顺反子表达载体所达到的表达水平相当的表达水平。
