Kettle A J, Carr A C, Winterbourn C C
Department of Pathology, Christchurch School of Medicine, New Zealand.
Free Radic Biol Med. 1994 Aug;17(2):161-4. doi: 10.1016/0891-5849(94)90111-2.
We used horseradish peroxidase and either scopoletin, homovanillic acid, or phenol red to measure hydrogen peroxide generated by human neutrophils. With these assays, superoxide dismutase significantly increased the amount of hydrogen peroxide detected. In contrast, it had no effect when the accumulation of hydrogen peroxide was measured with a hydrogen peroxide electrode. We propose that superoxide interferes with horseradish peroxidase-dependent assays so that hydrogen peroxide is underestimated. Thus, when using these assays, superoxide dismutase must be added to neutrophils to ensure that all the hydrogen peroxide they produce is detected.
我们使用辣根过氧化物酶以及东莨菪亭、高香草酸或酚红来测量人类中性粒细胞产生的过氧化氢。通过这些测定方法，超氧化物歧化酶显著增加了检测到的过氧化氢量。相比之下，当用过氧化氢电极测量过氧化氢积累量时，超氧化物歧化酶没有影响。我们认为超氧化物会干扰依赖辣根过氧化物酶的测定方法，从而导致过氧化氢被低估。因此，在使用这些测定方法时，必须向中性粒细胞中添加超氧化物歧化酶，以确保检测到它们产生的所有过氧化氢。
