Michael R D, Priscilla A S
Post-graduate and Research Department of Zoology, American College, Madurai, India.
Indian J Exp Biol. 1994 Jul;32(7):474-7.
Influence of time of antigen administration on antibody response to bovine serum albumin in the fish, O. mossambicus and variations in serum antibody titre during a 24 hr cycle are reported. Significant variations were observed in magnitude of antibody response in groups of fishes immunized at different times of the day. Magnitude of the response was maximal in fish immunized at 16 hr and minimal in those immunized at 4 hr. Serum antibody titres in a 24 hr study also varied markedly with general elevation during day time, reaching the maximum at 12 hr and depression during night with the lowest titre at 4 hr. These results can be positively correlated to diel variation in number of leucocytes or proportion of lymphocytes which in turn is possibly correlated (negatively) to the periodicity in plasma corticosteroid level.
报告了抗原接种时间对莫桑比克罗非鱼体内牛血清白蛋白抗体反应的影响以及24小时周期内血清抗体滴度的变化。在一天中不同时间免疫的鱼群中，观察到抗体反应强度存在显著差异。反应强度在16时免疫的鱼中最大，在4时免疫的鱼中最小。在一项24小时的研究中，血清抗体滴度也有明显变化，白天总体升高，在12时达到最高，夜间降低，在4时滴度最低。这些结果可能与白细胞数量或淋巴细胞比例的昼夜变化呈正相关，而白细胞数量或淋巴细胞比例的昼夜变化又可能（负）与血浆皮质类固醇水平的周期性相关。
