Dhasarathan Pallvarnanathasamy, Pugazhenthi M, Valivittan K
Prathyusah Institute of Technology and Management, Thiruvallur, India.
Department of Biotechnology, St Peter's University, Avadi, Chennai, India.
Cent Eur J Immunol. 2014;39(3):323-6. doi: 10.5114/ceji.2014.45942. Epub 2014 Oct 14.
From the Aeromonas hydrophila strain, five different types of antigens such as heat killed antigen, whole cell antigen, heat killed antigen with antiserum, whole cell antigen with antiserum and nucleotide antigens were prepared and injected into the experimental fish (Catla catla) groups for the study of immunomodulation. Analysis of immunogenicity of antigens against the fish Catla catla was estimated. The A. hydrophila produced β hemolytic pattern on the blood agar plate. B lymphocyte counts using rosette forming assay revealed a significant decrement in pathogens exposed fishes as compared to controls. Fishes exposed to pathogenic strains (1/10(th) sublethal concentration) for 3 weeks showed a reduction in PFC. The effect or pathogenic antigens in direct spleenic plaque forming cells (1 g M producing cells) showed a reduction in the secondary plaque forming cell in the first 3 weeks and a time- and dose-dependent decrease in primary and secondary PFC response. A remarkable observation enhancement in B cell production due to immune complex of antigens was noted in the present study. The enhancement of this type of immune responses confirms the potential of immune complexes to be used as vaccines.
从嗜水气单胞菌菌株中制备了五种不同类型的抗原，即热灭活抗原、全细胞抗原、热灭活抗原与抗血清混合物、全细胞抗原与抗血清混合物以及核苷酸抗原，并将其注射到实验鱼（印度鲮）组中，用于免疫调节研究。评估了这些抗原对印度鲮的免疫原性。嗜水气单胞菌在血琼脂平板上呈现β溶血模式。采用玫瑰花结形成试验进行的B淋巴细胞计数显示，与对照组相比，暴露于病原体的鱼的B淋巴细胞数量显著减少。暴露于致病菌株（1/10亚致死浓度）3周的鱼的PFC减少。致病抗原对直接脾集落形成细胞（1g M产生细胞）的影响显示，在前3周次级集落形成细胞减少，初级和次级PFC反应呈时间和剂量依赖性降低。在本研究中，注意到由于抗原免疫复合物导致的B细胞产生显著增强。这种类型免疫反应的增强证实了免疫复合物用作疫苗的潜力。
