Wolff H, Mögele T, Bezold G, Bollmann W, Brückner T, Noss U, Meurer M
Dermatologische Klinik und Poliklinik, Ludwig-Maximilians-Universität, München.
Hautarzt. 1994 Sep;45(9):605-10. doi: 10.1007/s001050050136.
The purpose of the study was to identify semen parameters relevant for in vitro fertilization (IVF). A total of 151 ejaculates from 130 patients were analyzed. There was a significant association between successful IVF, high sperm concentration and good sperm motility. Progressive sperm motility correlated best with successful fertilization of oocytes (r = +0.427) and was the only parameter significantly linked to pregnancy. Very low values were sufficient for fertilization in vitro: a sperm concentration of 4.5 x 10(6)/ml and a progressive sperm motility of 20%. The results of analysis of fructose, citrate and neutral alpha-glucosidase as markers of seminal vesicle, prostate and epididymal functions were not different in fertilizing and non-fertilizing semen samples. Moderate increases in granulocyte elastase levels, between 250 and 1000 ng/ml, did not influence IVF. However, two patients with genital tract inflammation (PMN-elastase > 1000 ng/ml) did not fertilize despite excellent semen parameters. These results stress the importance of andrological factors for in vitro fertilization outcome.
该研究的目的是确定与体外受精（IVF）相关的精液参数。对130名患者的总共151份射精样本进行了分析。体外受精成功与高精子浓度和良好的精子活力之间存在显著关联。进行性精子活力与卵母细胞的成功受精相关性最佳（r = +0.427），并且是唯一与妊娠显著相关的参数。非常低的值就足以进行体外受精：精子浓度为4.5×10⁶/ml，进行性精子活力为20%。作为精囊、前列腺和附睾功能标志物的果糖、柠檬酸盐和中性α-葡萄糖苷酶的分析结果在可受精和不可受精的精液样本中并无差异。粒细胞弹性蛋白酶水平在250至1000 ng/ml之间的适度升高并不影响体外受精。然而，两名患有生殖道炎症（PMN-弹性蛋白酶>1000 ng/ml）的患者尽管精液参数极佳却未实现受精。这些结果强调了男科因素对体外受精结果的重要性。
