Khattar M M, Begg K J, Donachie W D
Institute of Cell and Molecular Biology, University of Edinburgh, Scotland.
J Bacteriol. 1994 Dec;176(23):7140-7. doi: 10.1128/jb.176.23.7140-7147.1994.
The product of the ftsW gene has been identified as a polypeptide that, like the related RodA protein, shows anomalous mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. FtsW is produced at low levels that can be increased by altering the translation initiation region of the mRNA. Overproduction of FtsW strongly inhibits cell growth. A new mutant allele, ftsW201, causes a temperature-dependent block in the initiation stage of cell division which is similar to the division block in ftsZ mutants. The block in initiation of division in the ftsW201 allele is shown to be independent of FtsZ or the FtsZ inhibitor, SulA. In addition, the ftsW201 mutant is hypersensitive to overproduction of the division initiation protein FtsZ at the permissive temperature. Our results suggest a role for FtsW in an early stage of division which may involve an interaction with FtsZ.
ftsW基因的产物已被鉴定为一种多肽，它与相关的RodA蛋白一样，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中表现出异常迁移率。FtsW的产生水平较低，可通过改变mRNA的翻译起始区域来提高。FtsW的过量表达强烈抑制细胞生长。一个新的突变等位基因ftsW201在细胞分裂起始阶段导致温度依赖性阻断，这与ftsZ突变体中的分裂阻断相似。ftsW201等位基因在分裂起始时的阻断被证明独立于FtsZ或FtsZ抑制剂SulA。此外，ftsW201突变体在允许温度下对分裂起始蛋白FtsZ的过量表达高度敏感。我们的结果表明FtsW在分裂早期发挥作用，这可能涉及与FtsZ的相互作用。
