Bi E, Lutkenhaus J
Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City 66103.
J Bacteriol. 1993 Feb;175(4):1118-25. doi: 10.1128/jb.175.4.1118-1125.1993.
Immunoelectron microscopy was used to assess the effects of inhibitors of cell division on formation of the FtsZ ring in Escherichia coli. Induction of the cell division inhibitor SulA, a component of the SOS response, or the inhibitor MinCD, a component of the min system, blocked formation of the FtsZ ring and led to filamentation. Reversal of SulA inhibition by blocking protein synthesis in SulA-induced filaments led to a resumption of FtsZ ring formation and division. These results suggested that these inhibitors block cell division by preventing FtsZ localization into the ring structure. In addition, analysis of min mutants demonstrated that FtsZ ring formation was also associated with minicell formation, indicating that all septation events in E. coli involve the FtsZ ring.
免疫电子显微镜被用于评估细胞分裂抑制剂对大肠杆菌中FtsZ环形成的影响。细胞分裂抑制剂SulA（SOS反应的一个组成部分）或抑制剂MinCD（min系统的一个组成部分）的诱导，会阻止FtsZ环的形成并导致细胞丝状化。通过在SulA诱导的丝状体中阻断蛋白质合成来逆转SulA抑制作用，会导致FtsZ环的形成和细胞分裂的恢复。这些结果表明，这些抑制剂通过阻止FtsZ定位到环结构中来阻断细胞分裂。此外，对min突变体的分析表明，FtsZ环的形成也与微细胞的形成有关，这表明大肠杆菌中的所有隔膜形成事件都涉及FtsZ环。
