Laird S M, Tuckerman E, Li T C, Bolton A E
Division of Biomedical Sciences, Sheffield Hallam University, UK.
Hum Reprod. 1994 Jul;9(7):1339-43. doi: 10.1093/oxfordjournals.humrep.a138706.
The effect of interleukin 1 (IL1) and placental protein 14 (PP14) on the production of interleukin 6 (IL6) by cultured human endometrial epithelial cells prepared from endometrial biopsy material obtained at different stages in the menstrual cycle was investigated. Basal IL6 production by cells prepared from proliferative endometrium was greater than that produced by cells prepared from secretory endometrium (7.3 +/- 0.3 and 1.1 +/- 0.2 ng/well/24 h respectively, P < 0.001). IL1 (0.025-2.5 ng/ml) caused a dose-dependent increase in IL6 production by cells prepared from both proliferative and secretory endometrium, but cells prepared from secretory endometrium responded to a lower concentration of IL1 than those prepared from proliferative endometrium. IL-1-stimulated IL6 production by epithelial cells prepared from secretory endometrium typically reached 10 times basal values, while in cells prepared from proliferative endometrium stimulated levels were approximately twice the basal values. PP14 (1-50 micrograms/ml) also caused a dose-dependent increase in IL6 production by epithelial cells prepared from secretory endometrium, but had no effect on IL6 production by cells prepared from proliferative endometrium. Even in secretory cells PP14 was less effective than IL1 at stimulating IL6 production, with stimulated levels only reaching twice the basal values. This suggests that PP14 and IL1 act via different mechanisms in the stimulation of IL6 production. The results show that IL6 production by human endometrial epithelial cells is stimulated by other immunomodulatory peptides and this may be part of the network of such peptides in the endometrium which may influence embryo implantation.
研究了白细胞介素1（IL1）和胎盘蛋白14（PP14）对从月经周期不同阶段获取的子宫内膜活检材料制备的培养人子宫内膜上皮细胞产生白细胞介素6（IL6）的影响。增殖期子宫内膜制备的细胞基础IL6产量高于分泌期子宫内膜制备的细胞（分别为7.3±0.3和1.1±0.2 ng/孔/24小时，P<0.001）。IL1（0.025 - 2.5 ng/ml）使增殖期和分泌期子宫内膜制备的细胞IL6产量呈剂量依赖性增加，但分泌期子宫内膜制备的细胞对IL1的反应浓度低于增殖期子宫内膜制备的细胞。分泌期子宫内膜制备的上皮细胞经IL - 1刺激的IL6产量通常达到基础值的10倍，而增殖期子宫内膜制备的细胞刺激水平约为基础值的两倍。PP14（1 - 50微克/毫升）也使分泌期子宫内膜制备的上皮细胞IL6产量呈剂量依赖性增加，但对增殖期子宫内膜制备的细胞IL6产量无影响。即使在分泌期细胞中，PP14刺激IL6产生的效果也不如IL1，刺激水平仅达到基础值的两倍。这表明PP14和IL1在刺激IL6产生中通过不同机制起作用。结果表明，人子宫内膜上皮细胞产生IL6受到其他免疫调节肽的刺激，这可能是子宫内膜中此类肽网络的一部分，可能影响胚胎着床。
