Fukuda M, Azuma C, Kanai T, Koyama M, Kimura T, Saji F
Department of Obstetrics and Gynecology, Osaka University Medical School, Japan.
Int J Gynaecol Obstet. 1995 Jun;49(3):305-10. doi: 10.1016/0020-7292(95)02366-k.
To examine the expression of interleukin-1 (IL-1) and IL-1 receptor antagonist (IL-1ra) in the human endometrium in the follicular and luteal phases.
The concentrations of IL-1alpha and IL-1beta in the culture supernatants of endometrial cells were determined by enzyme-linked immunosorbent assay. Transcription of the IL-1ra gene in the endometrium was investigated by reverse polymerase chain reaction (PCR). Human endometrium was immunohistochemically stained using a monoclonal antibody specific to IL-1ra.
The concentrations of IL-1alpha and IL-1beta in the culture supernatants were 11 and 55 pg/ml, respectively, in the follicular phase, and 10 and 40 pg/ml, respectively, in the luteal phase. The concentration of IL-1ra was 465 pg/ml in the follicular phase and 1710 pg/ml in the luteal phase. Densitometric analysis of the reverse PCR products showed that the expression of IL-1ra mRNA was increased in endometrial cells in the luteal phase. Immunohistochemical staining revealed that epithelial cells were the main source of IL-1ra in human endometrium.
Human endometrial cells produce IL-1 (mainly IL-1beta) and IL-1ra. The level of IL-1ra production in human endometrial epithelial cells was greater in the luteal phase than in the follicular phase due to the increased transcription of the IL-1ra gene.
检测人子宫内膜在卵泡期和黄体期白细胞介素-1（IL-1）及IL-1受体拮抗剂（IL-1ra）的表达。
采用酶联免疫吸附测定法测定子宫内膜细胞培养上清液中IL-1α和IL-1β的浓度。通过逆转录聚合酶链反应（PCR）研究子宫内膜中IL-1ra基因的转录情况。使用特异性针对IL-1ra的单克隆抗体对人子宫内膜进行免疫组织化学染色。
卵泡期培养上清液中IL-1α和IL-1β的浓度分别为11 pg/ml和55 pg/ml，黄体期分别为10 pg/ml和40 pg/ml。卵泡期IL-1ra的浓度为465 pg/ml，黄体期为1710 pg/ml。逆转录PCR产物的光密度分析显示，黄体期子宫内膜细胞中IL-1ra mRNA的表达增加。免疫组织化学染色显示，上皮细胞是人类子宫内膜中IL-1ra的主要来源。
人子宫内膜细胞产生IL-1（主要是IL-1β）和IL-1ra。由于IL-1ra基因转录增加，人子宫内膜上皮细胞中IL-1ra的产生水平在黄体期高于卵泡期。
