Stewart J A, Bulmer J N, Murdoch A P
Centre for Reproductive Medicine, Royal Victoria Infirmary, Newcastle upon Tyne, UK.
J Clin Pathol. 1998 Feb;51(2):121-6. doi: 10.1136/jcp.51.2.121.
Stromal leucocyte populations in human endometrium comprise T cells, macrophages, and phenotypically unusual endometrial granulated lymphocytes. Their proportions vary during the menstrual cycle and, in particular, endometrial granulated lymphocytes increase in number in the late secretory phase. The stimulus responsible for these cyclical changes is unknown but it is likely that the steroid hormones oestrogen and progesterone play a role.
To define further the expression of steroid hormone receptors by leucocytes in non-pregnant and pregnant human endometrium.
Frozen and paraffin wax embedded sections of endometrium from non-pregnant women and early pregnancy decidua were labelled using single and double immunohistochemical techniques with monoclonal antibodies directed against oestrogen and progesterone receptors and various leucocyte subpopulations.
Despite the prominence of CD56 positive endometrial granulated lymphocytes in late secretory phase endometrium and early pregnancy decidua, double immunohistochemical labelling showed no evidence of expression of either progesterone or oestrogen receptors by these cells or other endometrial leucocyte populations.
Rather than acting directly, steroid hormones are likely to influence endometrial leucocyte populations indirectly via products of endometrial stromal or epithelial cells that express steroid hormone receptors.
人类子宫内膜中的基质白细胞群体包括T细胞、巨噬细胞和表型异常的子宫内膜颗粒淋巴细胞。它们的比例在月经周期中有所变化，特别是在分泌晚期，子宫内膜颗粒淋巴细胞数量增加。导致这些周期性变化的刺激因素尚不清楚，但雌激素和孕激素等类固醇激素可能起了作用。
进一步明确非妊娠和妊娠人类子宫内膜中白细胞类固醇激素受体的表达情况。
使用针对雌激素和孕激素受体以及各种白细胞亚群的单克隆抗体，通过单重和双重免疫组织化学技术，对非妊娠女性子宫内膜和早孕蜕膜的冷冻及石蜡包埋切片进行标记。
尽管CD56阳性子宫内膜颗粒淋巴细胞在分泌晚期子宫内膜和早孕蜕膜中占主导地位，但双重免疫组织化学标记显示，这些细胞或其他子宫内膜白细胞群体均未表达孕激素或雌激素受体。
类固醇激素可能并非直接发挥作用，而是通过表达类固醇激素受体的子宫内膜基质或上皮细胞产物间接影响子宫内膜白细胞群体。
