Maitani T, Kubota H, Hori N, Yoshihira K, Takeda M
National Institute of Hygienic Sciences, Tokyo, Japan.
J Appl Toxicol. 1994 Jul-Aug;14(4):257-61. doi: 10.1002/jat.2550140403.
The correlation between the in vivo tissue distribution or the urinary excretion of aluminium (Al) administered with four organic acids and the in vitro binding of Al to serum protein in the presence of the organic acids was studied: Aluminum was injected intraperitoneally into mice with an organic acid at a dose of 20 mg Al kg-1 (Al:ligand= 1:1 or 1:3). When the Al-citrate mixture was administered, hepatic and renal concentrations of Al at 3 h were low, while hepatic Al concentrations for the other three organic acids (malate, tartrate and isocitrate) were considerably higher. Compared to the sole administration of Al, urinary excretion of Al was at a high level in all organic acids. Aluminium added in vitro to control serum as an Al-citrate mixture was present only in a low-molecular-weight (LMW) fraction, while Al added with other organic acids was present in both the high-molecular-weight (HMW) and LMW fractions. Consequently, it was interpreted that Al in the LMW fractions was excreted into the urine and that a significant part of Al in the HMW fractions was transferred to the liver. The binding status of Al in serum observed in the in vitro study was also manifested in the in vivo samples obtained after the intravenous injection.
研究了四种有机酸与铝（Al）同时给药时铝在体内的组织分布或尿排泄情况，以及在有机酸存在下铝与血清蛋白的体外结合情况：将铝与一种有机酸以20 mg Al kg-1的剂量腹腔注射到小鼠体内（Al:配体 = 1:1或1:3）。当给予柠檬酸铝混合物时，3小时时肝脏和肾脏中的铝浓度较低，而其他三种有机酸（苹果酸、酒石酸和异柠檬酸）的肝脏铝浓度则高得多。与单独给予铝相比，所有有机酸中铝的尿排泄量都处于较高水平。体外添加到对照血清中的铝作为柠檬酸铝混合物仅存在于低分子量（LMW）部分，而与其他有机酸一起添加的铝则同时存在于高分子量（HMW）和LMW部分。因此，可以解释为LMW部分的铝排泄到尿液中，而HMW部分的很大一部分铝转移到了肝脏。体外研究中观察到的血清中铝的结合状态在静脉注射后获得的体内样本中也有体现。
