Gusmão R H, Mascarenhas J D, Gabbay Y B, Linhares A C
Serviço de Virologia Geral, Instituto Evandro Chagas, Fundação Nacional de Saúde, Belém, Pará, Brazil.
J Diarrhoeal Dis Res. 1994 Jun;12(2):129-32.
An atypical group-A rotavirus strain, with an electrophoretype displaying 5 segments in the first dsRNA size class, was detected among 3 hospitalized children less than 2 years old. Detection occurred initially 24 h after admission in a non-diarrhoeic child hospitalized because of acute respiratory infection. The second detection involved a child who occupied a different room within the same ward and who developed nosocomial diarrhoea 48 h later. A third case, also of hospital-acquired diarrhoea, was recorded in a child who occupied a bed in the same room as the second case and developed gastroenteritis 24 h following the second case's detection. In addition to the unusual, avian-like genomic profile, the strain was classified as serotype 2, based on a human VP7-specific enzyme-linked immunosorbent assay (ELISA). The question of whether these events reflect either a genomic rearrangement of a human rotavirus strain or a possible interspecies transmission will be further investigated through hybridization assays.
在3名2岁以下住院儿童中检测到一株非典型A组轮状病毒毒株，其电泳型在首个双链RNA大小类别中显示有5个片段。检测最初是在一名因急性呼吸道感染住院的非腹泻儿童入院24小时后进行的。第二次检测涉及一名在同一病房不同房间的儿童，该儿童在48小时后出现医院感染性腹泻。第三例同样是医院获得性腹泻，发生在与第二例同住一个房间的一名儿童身上，该儿童在第二例检测后24小时出现肠胃炎。除了这种不寻常的、类似禽源的基因组特征外，根据人VP7特异性酶联免疫吸附测定（ELISA），该毒株被分类为2型。这些事件是反映人轮状病毒毒株的基因组重排还是可能的种间传播，这个问题将通过杂交试验进一步研究。
